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125iodine 125i

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125Iodine (125I) is a radioactive isotope of iodine used in various laboratory applications. It has a half-life of approximately 60 days and emits gamma radiation. 125I is commonly used as a tracer in research and diagnostic procedures, but its specific applications and intended use should be determined by the user.

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Lab products found in correlation

Non specific mouse igg, by Jackson ImmunoResearch (4 mentions) Iodogen pre coated tubes, by PerkinElmer (4 mentions) Secondary goat anti mouse igg, by Jackson ImmunoResearch (2 mentions) Monoclonal mouse anti human icam 1 clone r6.5, by American Type Culture Collection (2 mentions) Phycoerythrin labeled monoclonal mouse anti human icam 1 clone lb 2, by Santa Cruz Biotechnology (2 mentions) Alexa fluor 350 labeled goat anti mouse igg, by Thermo Fisher Scientific (2 mentions) Green fluoresbrite polystyrene particles, by Polysciences (2 mentions) Porous transwell inserts, by Thermo Fisher Scientific (2 mentions) Human sicam 1 elisa kits, by Thermo Fisher Scientific (2 mentions) Mmp 9 inhibitor 1, by Merck Group (2 mentions) Mmp 2 inhibitor 1, by Merck Group (2 mentions) Cell culture media and supplements, by Corning (1 mentions) Hrp linked anti rabbit igg, by GE Healthcare (1 mentions) Rabbit anti human gapdh, by Santa Cruz Biotechnology (1 mentions) Fluorescently labeled secondary antibody, by Jackson ImmunoResearch (1 mentions) Pre cast 4 15 polyacrylamide gels, by Bio-Rad (1 mentions) Pierce iodination tubes, by Thermo Fisher Scientific (1 mentions) Pvdf membrane, by Pall Corporation (1 mentions) Mouse igg, by Jackson ImmunoResearch (1 mentions) Polystyrene beads, by Polysciences (1 mentions)

5 protocols using 125iodine 125i

1

Monoclonal Anti-ICAM-1 Binding Assay

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Monoclonal mouse anti-human ICAM-1 (anti-ICAM) was clone R6.5 (American Type Culture Collection; Manassas, VA). Non-specific mouse IgG and secondary goat anti-mouse IgG were from Jackson Immunoresearch (West Grove, PA). Dextranase (Dxase) from Penicillium janthinellum was from Sigma Aldrich (St. Louis, MO). Fluoresbrite® polystyrene latex particles were from Polysciences (Warrington, PA). 125Iodine (125I) and Iodogen pre-coated tubes were purchased from PerkinElmer (Waltham, MA) and Thermo Fisher Scientific (Waltham, MA), respectively. Cell culture media and supplements were from Cellgro (Manassas, VA), Gibco BRL (Grand Island, NY), or Sigma Aldrich (St. Louis, MO). Unless otherwise noted, all other reagents were from Sigma Aldrich (St. Louis, MO).
Hsu J., Hoenicka J, & Muro S. (2014). Targeting, Endocytosis, and Lysosomal Delivery of Active Enzymes to Model Human Neurons by ICAM-1-Targeted Nanocarriers. Pharmaceutical research, 32(4), 1264-1278.
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2

Quantifying Leukocyte-Endothelial Interactions

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Monoclonal mouse anti‐human ICAM‐1 (clone R6.5) was from ATCC (Manassas, VA) and phycoerythrin‐labeled monoclonal mouse anti‐human ICAM‐1 (clone LB‐2) was from Santa Cruz Biotechnology (Dallas, TX). Alexa Fluor 350‐labeled goat anti‐mouse IgG was from Invitrogen (Carlsbad, CA). Nonspecific mouse IgG was from Jackson ImmunoResearch (West Grove, PA). Green Fluoresbrite® polystyrene particles (100 nm in diameter) were from Polysciences (Warrington, PA). Porous transwell inserts (1.0 µm‐pore size) were from Thermo Fisher Scientific (Waltham, MA). Human sICAM‐1 ELISA kits were from Invitrogen (Carlsbad, CA). 125Iodine (125I) and Iodogen pre‐coated tubes were from PerkinElmer (Waltham, MA) and Thermo Fisher Scientific (Waltham, MA), respectively. MMP‐9 Inhibitor I and MMP‐2 Inhibitor I were from EMD Millipore (Billerica, MA). Unless specified, all other reagents were from Sigma‐Aldrich (St. Louis, MO).
Manthe R.L, & Muro S. (2017). ICAM‐1‐targeted nanocarriers attenuate endothelial release of soluble ICAM‐1, an inflammatory regulator. Bioengineering & Translational Medicine, 2(1), 109-119.
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3

ICAM-1 Immunolabeling Protocol

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Monoclonal mouse anti-human ICAM-1 (clone R6.5) was from ATCC (Manassas, VA) and phycoerythrin-labeled monoclonal mouse anti-human ICAM-1 (clone LB-2) was from Santa Cruz Biotechnology (Dallas, TX). Alexa Fluor 350-labeled goat anti-mouse IgG was from Invitrogen (Carlsbad, CA). Non-specific mouse IgG was from Jackson ImmunoResearch (West Grove, PA). Green Fluoresbrite® polystyrene particles (100 nm in diameter) were from Polysciences (Warrington, PA). Porous transwell inserts (1.0 µm-pore size) were from Thermo Fisher Scientific (Waltham, MA). Human sICAM-1 ELISA kits were from Invitrogen (Carlsbad, CA). 125Iodine (125I) and Iodogen pre-coated tubes were from PerkinElmer (Waltham, MA) and Thermo Fisher Scientific (Waltham, MA), respectively. MMP-9 Inhibitor I and MMP-2 Inhibitor I were from EMD Millipore (Billerica, MA). Unless specified, all other reagents were from Sigma-Aldrich (St. Louis, MO).
Manthe R.L, & Muro S. (2017). ICAM‐1‐targeted nanocarriers attenuate endothelial release of soluble ICAM‐1, an inflammatory regulator. Bioengineering & Translational Medicine, 2(1), 109-119.
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4

Monoclonal Anti-ICAM-1 Binding Assay

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Monoclonal mouse anti-human ICAM-1 (anti-ICAM) was clone R6.5 (ATCC; Manassas, VA). Non-specific mouse IgG and secondary goat anti-mouse IgG were from Jackson Immunoresearch (West Grove, PA). Fluoresbrite® polystyrene latex particles (not functionalized) were from Polysciences (Warrington, PA). 125Iodine (125I) and Iodogen pre-coated tubes were purchased from PerkinElmer (Waltham, MA) and Thermo Fisher Scientific (Waltham, MA), respectively. Unless otherwise noted all other reagents were from Sigma Aldrich (St. Louis, MO).
Hsu J., Rappaport J, & Muro S. (2014). Specific Binding, Uptake, and Transport of ICAM-1-Targeted Nanocarriers Across Endothelial and Subendothelial Cell Components of the Blood-Brain Barrier. Pharmaceutical research, 31(7), 1855-1866.
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5

Antibody and Reagent Sources

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Mouse anti-human ICAM-1 clone R6.5 (anti-ICAM) was from ATCC (Manassas, VA). Mouse IgM anti-ceramide was from Sigma-Aldrich (Saint Louis, MO). Rabbit anti-human PKC (H-300), rabbit anti-human GAPDH, and FITC-labeled goat anti-mouse IgM were from Santa Cruz Biotechnology (Dallas, TX). Rabbit anti-phosphorylated human PKCα (phospho T638) was from Abcam (Cambridge, United Kingdom). HRP-linked anti-rabbit IgG was from GE Healthcare (Pittsburgh, PA). Mouse IgG and fluorescently-labeled secondary antibodies were from Jackson Immunoresearch (West Grove, PA). Polystyrene beads were from Polysciences (Warrington, PA). 125Iodine (125I) was from Perkin-Elmer (Waltham, MA) and Pierce iodination tubes were from Thermo Scientific (Rockford, IL). Precast 4–15% polyacrylamide gels were from Biorad (Hercules, CA) and PVDF membranes were from Pall Life Sciences (Port Washington, NY). Unless noted, all other reagents were from Sigma-Aldrich.
Serrano D., Manthe R.L., Paul E., Chadha R, & Muro S. (2016). How carrier size and valency modulate receptor-mediated signaling: understanding the link between binding and endocytosis of ICAM-1-targeted carriers. Biomacromolecules, 17(10), 3127-3137.
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