A schematic of the optical layout of the O-PTIR system is shown in Figure S1 . O-PTIR and concomitant Raman spectroscopy were carried out using a mIRage IR microscope (Photothermal Spectroscopy Corporation, Santa Barbara, CA) integrated with a four-module-pulsed quantum cascade laser (QCL) system, with a tunable range from 1890 to 790 cm−1. The IR laser repetition rate was set at ca. 110 kHz at 300 ns per pulse. The system is also equipped with a tunable OPO-pulsed infrared laser to cover the higher wavenumber region, with a tunable range from 3593 to 2693 cm−1. The system is coupled to a Horiba Scientific iHR-320 Imaging spectrometer that we have repurposed and fitted to the mIRage system. This has a 100 μm slit width and a grating of 600, 1200, and 1800 l/mm, all blazed at 500 nm. The key difference between this and the commercial variant available from Photothermal at the time is that the charge-coupled device (CCD) camera is front-illuminated with a pixel size of 26, whereas the commercial system was back-illuminated but with a 14 μm pixel size. The larger pixel size resulted in approximately a factor of 2 improvement in the signal-to-noise ratio of the Raman spectra.
Ihr320 imaging spectrometer
Manufactured by Horiba
Sourced in Japan
The IHR320 is an imaging spectrometer designed for high-resolution spectral analysis. It features a compact and robust design, with a wavelength range covering the UV, visible, and near-infrared regions. The IHR320 is capable of providing detailed spectral information by dispersing light onto a 2D detector.
Automatically generated - may contain errors
4 protocols using ihr320 imaging spectrometer
1
Integrated O-PTIR and Raman Spectroscopy
2
Raman Spectroscopy of Nanorod-Analyte Interactions
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For Raman measurements, each nanorod sample was precipitated and redispersed either in a 10−5 M aqueous solution of Rhodamine 6G (Rh6G) or in a solution of promethazine (10−3 M in water), piroxicam (10−3 M in EtOH), furosemide (10−2 M in EtOH) or diclofenac (10−3 M in water).
Raman spectra were collected using a micro-Raman setup equipped with a He-Ne laser (Melles-Griot, Rochester, NY United States mod. 25LHP925) emitting at λ = 632.8 nm whose power on the sample was kept at 5 mW, ca. Samples were prepared by depositing each suspension on a germanium substrate. A back-scattering geometry was realized using the 50× long working distance objective of an OLYMPUS (Shinjuku, Tokyo, Japan) microscope MOD BX40, equipped with a digital camera. The scattered radiation was analyzed by an iHR320 imaging spectrometer Horiba Jobin-Yvon (Kyoto, Japan). The signal was dispersed by a 600 grooves/mm grating which allowed spectra acquisition in the 63–2691 cm−1 range and spectra were recorded as an average of 10 scans, each one accumulated within 30 s or 60 s integration time (depending on the sample) at 8 cm−1 resolution.
Raman spectra were collected using a micro-Raman setup equipped with a He-Ne laser (Melles-Griot, Rochester, NY United States mod. 25LHP925) emitting at λ = 632.8 nm whose power on the sample was kept at 5 mW, ca. Samples were prepared by depositing each suspension on a germanium substrate. A back-scattering geometry was realized using the 50× long working distance objective of an OLYMPUS (Shinjuku, Tokyo, Japan) microscope MOD BX40, equipped with a digital camera. The scattered radiation was analyzed by an iHR320 imaging spectrometer Horiba Jobin-Yvon (Kyoto, Japan). The signal was dispersed by a 600 grooves/mm grating which allowed spectra acquisition in the 63–2691 cm−1 range and spectra were recorded as an average of 10 scans, each one accumulated within 30 s or 60 s integration time (depending on the sample) at 8 cm−1 resolution.
3
Characterization of PPD and PVD Films
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Atomic force microscopy (AFM, Bruker Multimode 8) was used to examine the thickness and surface roughness of both PPD and PVD films. A tapping mode was employed at the ambient condition with various scan sizes and at a scan rate of 1 Hz. The detailed surface morphology was inspected by scanning electron microscopy (SEM, Hitachi S-4700 FESEM) with an acceleration voltage of 5 keV and a current of 10 µA. A thin layer of gold/palladium was sputtered (Cressington sputter coater 108) on the samples to enhance imaging resolution prior to SEM measurements. Optical properties (refractive index n, extinction coefficient k) of PPD and PVD films and dielectric layers were measured by UV-NIR spectroscopic ellipsometry (J.A. Woollam, M-2000). Olympus BX53 fluorescent microscope coupled Horiba iHR320 imaging spectrometer was utilized to record all the optical images and reflectance spectra of fabricated samples. A protected silver mirror (Thorlabs, PF10-03-P01) was used as a reference to calculate the relative reflectance of the samples, which has optical reflectance over 97.5% in the visible wavelength range.
4
Cryosectioning and Raman Spectroscopy of Cartilage
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cartilage samples were embedded in OCT prior to cryosectioning, cut (Leica CM1900 Cryostat) at 10 μm and mounted on a silicon substrate. Raman spectra were collected using a micro-Raman setup equipped with a solid-state laser at λ = 532 nm. Power was maintained at 8 mW to reduce photon damage to the samples during acquisition. A back-scattering geometry was realized using the 50× long working distance objective of an OLYMPUS microscope MOD BX40, equipped with a digital camera HORIBA, model Syncerity. The scattered radiation was analyzed by an iHR320 imaging spectrometer Horiba Jobin-Yvon. The signal was dispersed by a 1800 grooves/mm grating which allowed spectra acquisition in the 600-1720 cm -1 range. Spectra were recorded as an average of 10 scans, each one accumulated within 60s integration time, using at 5 cm -1 resolution.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!