Blood samples were immediately processed after collection as previously described (36 (link)). Whole blood was mixed with RNA Stabilization Reagent for Blood/Bone Marrow (Roche, Germany) for stabilization and stored at −20°C, in compliance with the instructions provided by the manufacturer. Next, samples were thawed at room temperature and mRNA was isolated using the mRNA Isolation Kit for Blood/Bone Marrow (Roche), according to the protocol provided by the manufacturer. Reverse Transcription (RT) was performed using a High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems).
Rna stabilization reagent for blood bone marrow
Manufactured by Roche
Sourced in Germany
The RNA Stabilization Reagent for Blood/Bone Marrow is a specialized solution designed to preserve and stabilize RNA molecules extracted from blood or bone marrow samples. This reagent helps to maintain the integrity of RNA, preventing degradation and ensuring accurate downstream analysis.
Automatically generated - may contain errors
2 protocols using rna stabilization reagent for blood bone marrow
1
Whole Blood mRNA Isolation and RT
2
Blood RNA Extraction and Reverse Transcription
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Blood samples were immediately processed after extraction. Whole blood was stabilized with RNA Stabilization Reagent for Blood/Bone Marrow (Roche, Germany) according to the manufacturer’s instructions and stored at −20°C. Then samples were thawed at room temperature, and mRNA was isolated using the mRNA Isolation Kit for Blood/Bone Marrow (Roche), according to the protocol provided by the manufacturer. Reverse transcription was carried out using Transcription First Strand cDNA Synthesis Kit (Roche).
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