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Rabbit anti ng2 chondroitin sulphate proteoglycan ng2

Manufactured by Merck Group

Rabbit anti-NG2 Chondroitin sulphate proteoglycan (NG2) is a laboratory reagent that detects the presence of the NG2 protein. NG2 is a chondroitin sulphate proteoglycan found on the surface of certain cells. This antibody can be used in various immunological techniques to identify and study cells expressing NG2.

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2 protocols using rabbit anti ng2 chondroitin sulphate proteoglycan ng2

1

Comprehensive Spinal Cord Immunostaining

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A one in forty-eight series of 40μm coronal spinal cord sections from each mouse were stained on slides using a modified immunofluorescence protocol for the following antibodies: rabbit anti-calbindin, 1:500, rabbit anti-calretinin, 1:500 and rabbit anti-parvalbumin 1:500, Swant, goat anti-Choline Acetyl transferase (ChAT), 1:50, Chemicon, rabbit anti- calcitonin gene related peptide (CGRP), 1:200, Enzo life sciences, rabbit anti-substance-P (SubP), 1:200, Chemicon, Rat anti-CD4,1:100, Bio-Rad, Rat anti- CD8, 1:100, Bio-Rad, rabbit anti-GFAP, 1:1000, DAKO, rat anti-mouse CD68, 1:400, Bio-Rad, rabbit anti-Olig2, 1:200, GeneTex, rabbit anti- NG2 Chondroitin sulphate proteoglycan (NG2), 1:200, Chemicon and rat anti-MBP, 1:500, Merck Millipore (See Supporting Methods).
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2

Multimodal Spinal Cord Immunostaining

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A one in forty‐eight series of 40µm coronal spinal cord sections from each mouse were stained on slides using a modified immunofluorescence protocol for the following antibodies: rabbit anti‐calbindin, 1:500, rabbit anti‐calretinin, 1:500 and rabbit anti‐parvalbumin 1:500, Swant, goat anti‐choline acetyl transferase (ChAT), 1:50, Chemicon, rabbit anti‐calcitonin gene‐related peptide (CGRP), 1:200, Enzo life sciences, rabbit anti‐substance‐P (SubP), 1:200, Chemicon, rat anti‐CD4,1:100, Bio‐Rad, rat anti‐CD8, 1:100, Bio‐Rad, rabbit anti‐GFAP, 1:1000, DAKO, rat anti‐mouse CD68, 1:400, Bio‐Rad, rabbit anti‐Olig2, 1:200, GeneTex, rabbit anti‐NG2 chondroitin sulphate proteoglycan (NG2), 1:200, Chemicon and rat anti‐MBP, 1:500, Merck Millipore (Data S1).
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