Mcf7 adr

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MCF7/ADR is a cell line derived from the human breast cancer cell line MCF-7. It exhibits resistance to the chemotherapeutic drug adriamycin (doxorubicin).

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6 protocols using mcf7 adr

Culturing Human Breast Cancer Cell Lines

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Human breast cancer cell lines MDA‐MB‐231, MCF7, MCF7/ADR, and benign breast epithelial cell line MCF‐10A were obtained from Chinese Type Culture Collection (Shanghai, China). MDA‐MB‐231 cells were cultured in Dulbecco's modified eagle medium (DMEM, Gibco, Grand Island, NY, USA) supplemented with 10% fetal bovine serum (FBS, Gibco) at 37℃ humidified incubator with 5% CO₂. MCF7 and MCF7/ADR cells were cultured in RPMI 1640 medium (Gibco) supplemented with 10% FBS at the same atmosphere mentioned above. MCF‐10A cell line was cultured in a 1:1 ratio of DMEM/F‐12 nutrient mixture (Gibco) medium supplemented with 10% FBS.

Jin X., Wei Y., Liu Y., Lu X., Ding F., Wang J, & Yang S. (2019). Resveratrol promotes sensitization to Doxorubicin by inhibiting epithelial‐mesenchymal transition and modulating SIRT1/β‐catenin signaling pathway in breast cancer. Cancer Medicine, 8(3), 1246-1257.

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Culturing Breast Cell Lines

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Human normal breast cells (MCF-10A), human breast cancer cells (MCF-7), and adriamycin-resistant breast cancer cells (MCF-7/ADR) were purchased from Shanghai Cell Research Institute, China. MCF-10A cells were cultured in DMEM/F-12 medium (Gibco, USA), and MCF-7 cells were cultured in 1640 medium (Gibco, USA), both containing 10% fetal bovine serum (FBS; Gibco, USA) and 1% penicillin-streptomycin; MCF-7/ADR cells were cultured in 1640 medium supplementary with 20% FBS and 1% penicillin-streptomycin. Incubation was done in an incubator containing 5% CO₂ and 95% humidity at 37°C.

Nong Q., Yu S., Hu H, & Hu X. (2021). Knockdown of lncRNA FOXD2-AS1 Inhibits Proliferation, Migration, and Drug Resistance of Breast Cancer Cells. Computational and Mathematical Methods in Medicine, 2021, 9674761.

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Breast Cancer Cell Lines and ROCK Inhibitor

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293T, MCF-10A (normal breast epithelial cell lines), MCF-7 (relative low metastatic breast cancer cell lines), MDA-MB-435 (relative median metastatic breast cancer cell lines) and MDA-MB-231 (relative high metastatic breast cancer cell lines) cells were purchased from the cell bank of the Chinese Academy of Sciences (Shanghai, China). Doxorubicin resistant MCF-7 cells (MCF-7-ADR) were purchased from KeyGen Biotech (Nanjing, China). MCF-10A, MCF-7 and MCF-7-ADR cells were cultured in Dulbecco's Modified Eagle Medium (Gibco, Grand Island, NY, USA), MDA-MB-435 cells were cultured in 1640 medium (Gibco,), MDA-MB-231 cells were cultured in L-15 medium (Gibco) with 10% fetal bovine serum, 80 U/ml penicillin and 0.08 mg/ml streptomycin at 37°C under humidified air with 5% CO₂. Y-27632 (B1293), a ROCK inhibitor, was purchased from ApexBio (Hsinchu City, Taiwan).

Zhou G., Liu X., Xiong B, & Sun Y. (2017). Homeobox B4 inhibits breast cancer cell migration by directly binding to StAR-related lipid transfer domain protein 13. Oncology Letters, 14(4), 4625-4632.

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Breast Cancer Cell Line Cultivation and Treatment

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Breast cancer cell lines T47D, BT549, MDA‐MB‐231, MCF‐7, and MCF‐7/ADR and benign breast epithelial cell line MCF‐10A were purchased from Chinese Type Culture Collection (Shanghai, China). MCF‐7 is doxorubicin‐sensitive cell line and MCF‐7/ADR is a human breast adenocarcinoma multidrug‐resistant cell line selected against doxorubicin. T47D, BT549, and MCF‐7/ADR cell lines were cultured in RPMI 1640 medium (Gibco, Grand Island, NY) supplemented with 10% heat‐inactivated fetal bovine serum (FBS; Gibco). MCF‐7 and MDA‐MB‐231 cell lines were cultured in Dulbecco's modified Eagle's medium (DMEM) (Gibco) supplemented with 10% heat‐inactivated FBS (FBS; Gibco). MCF‐10A cell line was cultured in a 1:1 ratio of DMEM and Ham's F‐12 nutrient mixture supplemented with 10% heat‐inactivated FBS and 1% penicillin–streptomycin, 10 μg/mL insulin (Humulin 30/70), 20 ng/mL epidermal growth factor (EGF), 500 ng/mL hydrocortisone, and 100 ng/mL cholera toxin. All cultures were grown at 37°C in 5% CO₂ atmosphere.
Doxorubicin (Dox; Adriamycin, ADR) was purchased from Sigma (St. Louis, MO). The PA‐MSHA used in the present study was purchased from Wanter Biopharma Company (Beijing, China), then scale‐diluted and stored at 4°C. Phosphate‐buffered saline (PBS 0.1 mol/L; Gibco) was used as control.

Wei Y., Liu D., Jin X., Gao P., Wang Q., Zhang J, & Zhang N. (2016). PA‐MSHA inhibits the growth of doxorubicin‐resistant MCF‐7/ADR human breast cancer cells by downregulating Nrf2/p62. Cancer Medicine, 5(12), 3520-3531.

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Breast Cancer Cell Lines Culture

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Human breast cancer cell lines, MCF-7, MDA-MB-468, MDA-MB-436, T-47D and MCF-7/Adr were obtained from American Type Culture Collection (ATCC, Manassas, USA). MCF-7, T-47D, MCF-7/Adr were cultured in RPMI-1640 medium containing 10% fetal bovine serum (Gibco) and 100 I.U./ml penicillin and 100 μg/ml streptomycin at 37 °C in a humidified atmosphere with 5% CO₂. MDA-MB-468 and MDA-MB-436 were cultured in DMEM medium containing 10% fetal bovine serum (Gibco) and 100 I.U./ml penicillin and 100 μg/ml streptomycin at 37 °C in a 5% CO2 air atmosphere as mentioned before.

Luo Y., He J., Yang C., Orange M., Ren X., Blair N., Tan T., Yang J.M, & Zhu H. (2017). UCHL1 promotes invasion of breast cancer cells through activating Akt signaling pathway. Journal of cellular biochemistry, 119(1), 691-700.

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Breast Cancer Cell Line Comparison

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The breast cancer cell lines MCF-7 and MDA-MB-231 were purchased from the China Academia Sinica Cell Repository (Shanghai). MCF-7 adriamycin resistant cells (MCF-7-Adr) were purchased from KeyGEN BioTECH (Nanjing, China). MCF-7 and MCF-7-Adr cells were cultured in DMEM medium (Gibco), and MDA-MB-231 cells were maintained in L-15 medium (Gibco) supplemented with 10% fetal bovine serum (FBS) (Gibco), 80 Units/ml penicillin and 0.08 mg/ml streptomycin at 37°C under humidified atmosphere with 5% CO 2 . The resistance of MCF-7-Adr cells were confirmed before using. Human recombinant VEGF-A (ab9571) was purchased from Abcam. The inhibitors of VEGF-A/NRP-1, EG00229 (HY-10799) and Wnt/β-catenin, Wnt-C59 (HY-15659) were purchased from Medchem Express.

Zhang L., Wang H., Li C., Zhao Y., Wu L., Du X, & Han Z. (2017). VEGF-A/Neuropilin 1 Pathway Confers Cancer Stemness via Activating Wnt/β-Catenin Axis in Breast Cancer Cells. Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 44(3).

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