Log In Sign up
The largest database of trusted experimental protocols

Rnaiso trizol reagent

Manufactured by Takara Bio
Visit Supplier
Sourced in Japan

RNAiso TRIzol reagent is a monophasic solution of phenol and guanidine isothiocyanate designed for the isolation of total RNA from various biological samples. It is a reagent used for the extraction and purification of RNA.

Automatically generated - may contain errors

Lab products found in correlation

Primescript rt master mix, by Takara Bio (4 mentions) Cfx96 real time pcr detection system, by Bio-Rad (2 mentions) Sybr fast qpcr mix, by Takara Bio (2 mentions) Applied biosystems 7500, by Thermo Fisher Scientific (1 mentions) Sybr green, by Takara Bio (1 mentions) Sybr premix ex taq 2, by Takara Bio (1 mentions)

Spelling variants of this product

TRIzol reagent (5030 citations) TRIzol (1653 citations) RNAiso reagent (525 citations) RNAiso (278 citations) Trizol reagent RNAiso Plus (23 citations)

4 protocols using rnaiso trizol reagent

1

Quantitative RT-PCR Analysis of Gene Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols
Total RNA was isolated using RNAiso TRIzol reagent (Takara) and reverse transcribed with PrimeScript RT Master Mix (Takara) according to the manufacturer's instructions. Then, SYBR Fast qPCR Mix (Takara) in a Bio‐Rad CFX96 Real‐Time PCR Detection System (Bio‐Rad) was used for qRT‐PCR. qRT‐PCR was performed in triplicate, and the results were normalized against GAPDH.
Wang L., Yuan Y., Wang J., Luo Y., Lan Y., Ge J., Li L., Liu F., Deng Q., Yan Z., Liang M., Wei S., Liu X., Wang Y., Ping Y., Shi Y., Yu S., Zhang X., Cui Y., Yao X., Feng H., Luo T, & Bian X. (2022). ASCL2 Maintains Stemness Phenotype through ATG9B and Sensitizes Gliomas to Autophagy Inhibitor. Advanced Science, 9(27), 2105938.
+ Open protocol
+ Expand
2

Quantitative RT-PCR Analysis of Gene Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols
Total RNA was isolated using RNAiso TRIzol reagent (Takara), and reverse-transcribed with PrimeScript RT Master Mix (Takara) according to the manufacturer’s instructions. Then a SYBR Fast qPCR Mix (Takara) in a Bio-Rad CFX96 Real-Time PCR Detection System (Bio-Rad) was used for qRT-PCR. qRT-PCR was performed in triplicate and the results were normalized against β-actin. The sequences of all primers for qRT-PCR are listed in Supplementary Table S1.
Ji C.D., Wang Y.X., Xiang D.F., Liu Q., Zhou Z.H., Qian F., Yang L., Ren Y., Cui W., Xu S.L., Zhao X.L., Zhang X., Wang Y., Zhang P., Wang J.M., Cui Y.H, & Bian X.W. (2018). Kir2.1 Interaction with Stk38 Promotes Invasion and Metastasis of Human Gastric Cancer by Enhancing MEKK2–MEK1/2–ERK1/2 Signaling. Cancer research, 78(11), 3041-3053.
+ Open protocol
+ Expand
3

Quantitative Gene Expression Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Total RNA was extracted using RNAiso Trizol reagent (Takara, Kyoto, Japan), PrimeScript™ RT Master Mix (Takara, Japan) was used for reverse transcription according to the manufacturer's instructions. cDNA was amplified and quantified by Applied Biosystems 7500 (Thermo Fisher, USA) using SYBR Green (TaKaRa, Japan). GAPDH was used as internal controls, Primers were synthesized and purified by RiboBio (Guangzhou, China) and the primers are listed in Additional file 5: Table S5. The fold changes were determined using the relative quantification 2-△△CT method.
Chen S., Wang B., Fu X., Liang Y., Chai X., Ye Z., Li R., He Y., Kong G., Lian J., Li X., Chen T., Zhang X., Qiu X., Tang X., Zhou K., Lin B, & Zeng J. (2021). ALKAL1 gene silencing prevents colorectal cancer progression via suppressing Sonic Hedgehog (SHH) signaling pathway. Journal of Cancer, 12(1), 150-162.
+ Open protocol
+ Expand
4

Quantitative RT-PCR for FPR2 Gene Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols
Total RNA was isolated using RNAiso TRIzol reagent (TAKARA, Kyoto, Japan) according to the manufacturer’s instructions. Reverse-transcription of RNA was performed in a final reaction volume of 20 μL containing 1000 ng of total RNA by using PrimeScript RT Master Mix (TAKARA, Kyoto, Japan). FPR2 mRNAs were detected by qRT-PCR with the SYBR Premix Ex TaqII (TAKARA, Kyoto, Japan). The sequences of all primers for RT-qPCR were presented in Table S2.
Hou X.L., Ji C.D., Tang J., Wang Y.X., Xiang D.F., Li H.Q., Liu W.W., Wang J.X., Yan H.Z., Wang Y., Zhang P., Cui Y.H., Wang J.M., Bian X.W, & Liu W. (2017). FPR2 promotes invasion and metastasis of gastric cancer cells and predicts the prognosis of patients. Scientific Reports, 7, 3153.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!