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The Zap-70 is a laboratory instrument designed for the detection and quantification of the Zap-70 protein in cell samples. It utilizes flow cytometry technology to analyze cellular expression of Zap-70, which is a key signaling molecule in T-cell activation. The Zap-70 instrument provides researchers with a reliable and efficient means of measuring this important biomarker.

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Lab products found in correlation

Ikzf1, by Thermo Fisher Scientific (2 mentions) Ikzf3 on targetplus sirna smartpools, by Thermo Fisher Scientific (2 mentions) Cell line nucleofector kit t solution, by Lonza (2 mentions) Streptavidin magnesphere paramagnetic particles, by Promega (1 mentions)

3 protocols using zap 70

1

siRNA Transfection for Zap-70, CRBN, IKZF1, and IKZF3

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For siRNA transfection, non-targeted, Zap-70, CRBN, IKZF1, and IKZF3 ON-Targetplus siRNA SMARTpools were purchased from Thermo Scientific (Lafayette, CO). siRNA transfection was carried out by Amaxa electroporation system using the “Cell Line Nucleofector® Kit T solution (Lonza, Koln, Germany). Sequences of small interference RNAs are listed in Supplementary Table S2.
Hideshima T., Ogiya D., Liu J., Harada T., Kurata K., Bae J., Massefski W, & Anderson K.C. (2020). Immunomodulatory drugs activate NK cells via both Zap-70 and cereblon-dependent pathways. Leukemia, 35(1), 177-188.
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2

siRNA Transfection for Zap-70, CRBN, IKZF1, and IKZF3

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For siRNA transfection, non-targeted, Zap-70, CRBN, IKZF1, and IKZF3 ON-Targetplus siRNA SMARTpools were purchased from Thermo Scientific (Lafayette, CO). siRNA transfection was carried out by Amaxa electroporation system using the “Cell Line Nucleofector® Kit T solution (Lonza, Koln, Germany). Sequences of small interference RNAs are listed in Supplementary Table S2.
Hideshima T., Ogiya D., Liu J., Harada T., Kurata K., Bae J., Massefski W, & Anderson K.C. (2020). Immunomodulatory drugs activate NK cells via both Zap-70 and cereblon-dependent pathways. Leukemia, 35(1), 177-188.
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3

Identifying Cellular Signaling Proteins

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Antibodies were purchased from Cell Signaling Technology [Yes (#3201), Lck (#2657), Zap70 (#2705, #2709), pZap70(Y319) (#2701), pZap70(Y493) (#2704)], Thermo Scientific [V5 Tag (#MA5-15253)], Streptavidin-Dylight650 (#84547), Millipore [T-cell receptor, clone C305 [#05-919)], Sigma Aldrich [Anti-Mouse IgG FITC antibody (#F0257)], and Rockland [Myc-tag (#600-432-381)]. Streptavidin Magnesphere Paramagnetic Particles (Promega #Z5481) were used for the recombinant SH2 pull-down and during the monobody selection.
Kükenshöner T., Schmit N.E., Bouda E., Sha F., Pojer F., Koide A., Seeliger M., Koide S, & Hantschel O. (2017). Selective Targeting of SH2 Domain–Phosphotyrosine Interactions of Src Family Tyrosine Kinases with Monobodies. Journal of Molecular Biology, 429(9), 1364-1380.
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