Chicken-anti-βgal (1:200, Immunology Consultants Laboratory), rabbit-anti-βgal (1:1000, Molecular Probes), mouse-anti-CycB (1:100, Developmental Studies Hybridoma Bank/DSHB), rat-anti-DEcad (1:50, DSHB), rabbit-anti-Dgo (1:100, Feiguin et al., 2001 (link)), rat-anti-Elav (1:50, DSHB), Mouse-anti-Fmi (1:10, DSHB), mouse-anti-GFP (1:1000, Roche), rabbit-anti-Nek2 (1:1000, Prigent et al., 2005 (link)), rat-anti-Pk (1:25; Strutt et al., 2013 (link)) mouse-anti-Ro (1:10, DSHB), mouse-anti-γTub (1:1000, Sigma), Rhodamine phalloidin (1:1000, Invitrogen), phalloidin-AF647 (1:1000, Jackson IRL), Hoechst 33342 (10mg/ml, 1:500). All fluorophore coupled secondaries were from Jackson IRL used at 1:200.
Mouse anti cycb
Manufactured by Developmental Studies Hybridoma Bank
Mouse anti-CycB is an antibody product developed and distributed by the Developmental Studies Hybridoma Bank. This antibody is specific to the Cyclin B protein, which plays a crucial role in the regulation of the cell cycle. The product is intended for use in research applications, but no further details on its intended use or applications are provided.
Automatically generated - may contain errors
Lab products found in correlation
Rhodamine phalloidin, by Thermo Fisher Scientific (1 mentions)
Mouse anti gfp, by Roche (1 mentions)
Rabbit anti β gal, by Thermo Fisher Scientific (1 mentions)
Mouse anti γtub, by Merck Group (1 mentions)
Alexa fluor 647 goat anti rabbit, by Thermo Fisher Scientific (1 mentions)
Hrp conjugated anti rabbit igg, by Jackson ImmunoResearch (1 mentions)
Mouse anti actin, by Merck Group (1 mentions)
4 protocols using mouse anti cycb
1
Immunofluorescence Labeling of Drosophila Tissues
2
Immunostaining of Drosophila Tissues
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Primary antibodies were mouse anti-CycB [Developmental Studies Hybridoma Bank (DSHB), F2F4; 1:15] and anti-DLG (DSHB, 4F3; 1:15), rabbit anti-NRXIV (Manzoor Bhat, University of Texas Health Science Center; 1:1000), anti-phospho-histone H3 (Ser10; EMD Millipore, #06-570; 1:400), rat anti-ELAV (DSHB, 7E8A10; 1:15) and guinea-pig anti-GFP (gift from Mary-Lou Pardue, Massachusetts Institute of Technology; 1:400). The GFP-Booster nanobody (Chromotek, #gba488) was used at 1:400. Secondary antibodies were Alexa Fluor 568 goat anti-mouse or anti-rabbit (1:1000; Life Technologies), Alexa Fluor 647 goat anti-rabbit (1:1000; Life Technologies), and RRX- and Cy5-conjugated to donkey/goat anti-rabbit or anti-rat (1:250; Jackson ImmunoResearch).
3
Protein Detection and Analysis Protocol
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Oocyte isolation, protein extract preparation, SDS-PAGE, filter transfer, antibody binding, protein detection, and membrane re-probing were done as previously described (Hara et al., 2017 (link)). All immunoblot analyses were repeated in at least two biological replicates.
Primary antibodies used in this study were guinea pig anti-GNU (1/5000 in TBS-T) (Lee et al., 2003 (link)), rabbit anti-BIC-C (1/2000 in TBS-T; from Paul Lasko, McGill University), guinea pig anti-GFP (1/5000 in TBS-T; a gift from Mary-Lou Pardue, MIT), rabbit anti-ME31B (1/10,000 in TBS-T) (Nakamura et al., 2001 (link)), rat anti-αTUB YOL1/34 (1/1000 in TBS-T; AbD Serotec, Raleigh), mouse anti-CycB (1/100, TBS-T; Developmental Studies Hybridoma Bank, Iowa City, IA), rat anti-MBP (1/2000 in Hikari Solution; Sigma-Aldrich, St Louis, MO), and rabbit anti-GST labeled with HRP (1/5000 in Hikari solution; MBL, Woburn, MA). Secondary antibodies used were HRP-conjugated anti-rabbit IgG, anti-guinea pig IgG, anti-mouse IgG, and anti-rat IgG (Jackson ImmunoResearch, West Grove, PA).
Primary antibodies used in this study were guinea pig anti-GNU (1/5000 in TBS-T) (Lee et al., 2003 (link)), rabbit anti-BIC-C (1/2000 in TBS-T; from Paul Lasko, McGill University), guinea pig anti-GFP (1/5000 in TBS-T; a gift from Mary-Lou Pardue, MIT), rabbit anti-ME31B (1/10,000 in TBS-T) (Nakamura et al., 2001 (link)), rat anti-αTUB YOL1/34 (1/1000 in TBS-T; AbD Serotec, Raleigh), mouse anti-CycB (1/100, TBS-T; Developmental Studies Hybridoma Bank, Iowa City, IA), rat anti-MBP (1/2000 in Hikari Solution; Sigma-Aldrich, St Louis, MO), and rabbit anti-GST labeled with HRP (1/5000 in Hikari solution; MBL, Woburn, MA). Secondary antibodies used were HRP-conjugated anti-rabbit IgG, anti-guinea pig IgG, anti-mouse IgG, and anti-rat IgG (Jackson ImmunoResearch, West Grove, PA).
4
Extracting Ovary Proteins for Western Blotting
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Extracts for western blotting were obtained from ovaries that were dissociated into individual eggs and egg chambers by treatment with collagenase. Stage 13 and stage 14 eggs were selectively enriched by repeated rounds of mixing and then pouring off the slower settling smaller egg chambers. Laid eggs were obtained from 0–2 hr collections from females fertilized by XO males. Mouse anti-CycB and mouse anti-CycA antibodies (Developmental Studies Hybridoma Bank) were used at 1/20 and 1/5, respectively. Rabbit anti PSTAIR antisera (Santa Cruz) was used at 1/1000 and mouse anti-Actin (Millipore) was used at 1/5000. HRP-conjugated secondary antibodies (Roche) were used at 1/7000 and detected by ECL.
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