Discovery series quantity one 1 d analysis software

Whole cells were fixed in 1.5% KMnO₄ for 20 min at room temperature, dehydrated by successive incubations in increasing concentrations of ethanol, and embedded in Poly/Bed 812 epoxy resin (Polysciences). Ultrathin sections were cut using an Ultra-Cut E Microtome (Reichert-Jung). Silver/gold thin sections from the embedded blocks were examined in a transmission electron microscope (JEM-2000FX; JEOL). For morphometric analysis of random electron microscopic sections of cells, 12- × 14-cm prints and 8- × 10-cm negatives of 35–40 cell sections of each strain at 24,000–29,000 magnification were scanned and converted to digitized images with a ScanJet 4400c (Hewlett-Packard) and Photoshop 6.0 software (Adobe Systems, Inc.). Quantitation of digitized images was performed using the Discovery Series Quantity One 1-D Analysis Software (Bio-Rad Laboratories).