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Antibodies for western blot analysis

Manufactured by Santa Cruz Biotechnology
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Antibodies for Western blot analysis. These antibodies are designed for use in Western blot techniques to detect and quantify specific proteins in a sample.

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Lab products found in correlation

Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions) Acridine orange, by Merck Group (1 mentions) 3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt, by Merck Group (1 mentions) Raw 264.7 murine macrophages, by Korean Cell Line Bank (1 mentions) 4 amino 5 methylamino 2 7 difluorofluorescein diacetate daf fm da, by Merck Group (1 mentions) Tnf α, by BD (1 mentions) Mouse il 6, by BD (1 mentions) Dialyzed fbs, by Atlanta Biologicals (1 mentions) Silibinin, by Merck Group (1 mentions) Tnf α, by R&D Systems (1 mentions) Fetal bovine serum (fbs), by Atlanta Biologicals (1 mentions) Streptomycin, by Sartorius (1 mentions) Amphotericin, by Sartorius (1 mentions) Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (1 mentions) Propidium iodide, by Merck Group (1 mentions) Nitro l arginine methyl ester l name, by Merck Group (1 mentions) Compound c, by Merck Group (1 mentions)

Spelling variants of this product

Western blot antibodies (4 citations) Peroxidase-conjugated secondary antibodies for Western blot analysis (4 citations) Secondary antibodies for western blot analysis (2 citations)

4 protocols using antibodies for western blot analysis

1

Murine Macrophage and Zebrafish Protocols

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RAW 264.7 murine macrophages were obtained from the Korean Cell Line Bank (KCLB, Seoul, South Korea). Adult zebrafish (to obtain the eggs) were purchased from a commercial fish seller South Korea. Dulbecco's Modified Eagle's Medium (DMEM), penicillin/streptomycin mixture and fetal bovine serum (FBS) were purchased from GIBCO INC. (NY, USA). LPS from Salmonella enterica, 4-amino-5-methylamino-2′,7′-difluorofluorescein diacetate (DAF-FM DA), 2′,7′-2′7′-dichlorodihydrofluorescein diacetate (DCFH2-DA), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), and acridine orange were purchased from Sigma, Aldrich, USA. Antibodies for Western blot analysis were purchased from Santa Cruz Biotechnology (USA). ELISA kits (PGE2, TNF-α, Mouse IL-1beta, and Mouse IL-6) were purchased from BD Biosciences (USA), eBioscience, Inc. (USA), and R&D Systems, Inc. All the solvents used during the extraction and fractionation were of analytical grade.
Fernando I.P., Lee W.W., Jayawardena T.U., Kang M.C., Ann Y.S., Ko C.I., Park Y.J, & Jeon Y.J. (2018). 3β-Hydroxy-Δ5-steroidal congeners from a column fraction of Dendronephthya puetteri attenuate LPS-induced inflammatory responses in RAW 264.7 macrophages and zebrafish embryo model. RSC Advances, 8(33), 18626-18634.
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2

Silibinin and 1,25D Effects on TNF-α

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1,25D and silibinin were purchased from Sigma (St. Louis, MO). 1,25D was dissolved in ethanol at 10−3M, and silibinin was dissolved in DMSO at 100 mM. TNF-α was purchased from R&D Biosystems (Minneapolis, MN) and was dissolved in PBS containing 0.1% BSA at 10 μg/0.5 ml. Fetal bovine serum (FBS) and dialyzed FBS were obtained from Atlanta Biologicals (Norcross, GA). Tissue culture supplies were purchased from Mediatech (Manassas, VA). Antibodies for Western blot analysis were obtained from Santa Cruz Biotechnology (Santa Cruz, CA).
Bhatia V, & Falzon M. (2015). Restoration of the anti-proliferative and anti-migratory effects of 1,25-dihydroxyvitamin D by silibinin in vitamin D-resistant colon cancer cells. Cancer letters, 362(2), 199-207.
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3

Cytotoxicity Assay with PectaSol-CMCP

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PectaSol-CMCP (ecoNugenics, Santa Rosa, CA) was prepared in a stock of 25 mg/mL
in H2O, stored at −20°C, thawed and diluted in a cell culture medium
immediately before treatment. Antibiotics (penicillin, streptomycin,
amphotericin) and a kit for XTT-based cell proliferation assay were obtained
from Biological Industries (Beit-HaEmek, Israel). Dulbecco’s modified Eagle’s
medium (DMEM) and fetal bovine serum (FBS) were purchased from Life Technologies
(Rehovot, Israel). Propidium iodide (PI) was obtained from Sigma-Aldrich (St.
Louis, MO). Antibodies for western blot analysis were obtained from Santa Cruz
Biotechnology (Santa Cruz, CA).
Conti S., Vexler A., Hagoel L., Kalich-Philosoph L., Corn B.W., Honig N., Shtraus N., Meir Y., Ron I., Eliaz I, & Lev-Ari S. (2018). Modified Citrus Pectin as a Potential Sensitizer for Radiotherapy in Prostate Cancer. Integrative Cancer Therapies, 17(4), 1225-1234.
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4

Investigating Vascular Biomarkers in Disease

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CoPP and SnPP were purchased from Frontier Scientific, Inc. (Logan, UT, USA). Norepinephrine (NE), acetylcholine (Ach), sodium nitroprusside (SNP), nitro-L-arginine methyl ester (L-NAME) and Compound C were purchased from Sigma (St. Louis, MO, USA). Antibodies for western blot analysis were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Adiponectin and high-sensitivity C-reactive protein (hs-CRP) assay kits were purchased from Uscn Life Science Inc. (Wuhan, China). HO-1 assay kit was purchased from Stressgen-Assay Design (Ann Arbor, MI, USA). NO and malondialdehyde (MDA) assay kits were purchased from Beyotime Biotechnology (Beijing, China). Serum TNF-α enzyme-linked immunosorbent assay (ELISA) kit was purchased from R&D Systems (Minneapolis, MN, USA). Serum levels of adiponectin and hs-CRP were measured by ELISA (Uscn Life Science Inc.).
Han F., Guo Y., Xu L., Hou N., Han F, & Sun X. (2015). Induction of Haemeoxygenase-1 Directly Improves Endothelial Function in Isolated Aortas from Obese Rats through the Ampk-Pi3k/Akt-Enos Pathway. Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 36(4).
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