General bacterial 16S rDNA gene profiles of fecal bacteria in three model groups and control group were generated using the D-Code universal mutation detection system (Bio-Rad, Hercules, CA).Briefly, gels were prepared using 30–70% denaturing gradients containing 8% polyacrylamide. The amplification products of universal primers GC357F and 518 R targeting bacterial 16 S rDNA-V3 region were loaded onto the gel and electrophoresed in 1x Tris-acetate-EDTA buffer (40 mM Tris, 20 mM acetic acid, 1 mM EDTA) (TAE).Gels were run at 60 V for 2.5 h firstly, and then were run at 90 v for 12 h (DGGE-2001, C.B.S. Scientific, San Diego, CA, USA), and stained with SYBRI (Invitrogen, Carlsbad, CA, USA). Gel bands were then visualized with a Typhoon FLA 9500 Molecular Imager (GE Healthcare, Pittsburgh, PA, USA). Community profiles were subjected to cluster analysis using QuantityOne software (version 4.2; BioRad). Dice’s band-matching coefficient and unweighted pair group method with arithmetic averages (UPGMA) were employed to analyze the results.
Sybr 1
Manufactured by Thermo Fisher Scientific
Sourced in United States, France
SYBR I is a fluorescent dye used for nucleic acid detection and quantification. It binds to double-stranded DNA, emitting a fluorescent signal that can be measured to determine the amount of DNA present in a sample.
Automatically generated - may contain errors
Lab products found in correlation
Dcode universal mutation detection system, by Bio-Rad (1 mentions)
Typhoon fla 9500 molecular imager, by GE Healthcare (1 mentions)
Quantity one software, by Bio-Rad (1 mentions)
Sodium borate, by Thermo Fisher Scientific (1 mentions)
Streptavidin magnetic beads, by New England Biolabs (1 mentions)
Rnase inhibitor, by Takara Bio (1 mentions)
Cis bis 2 2 bipyridine dichlororuthenium 2 2 2 bipyridine 4 4 dicarboxylic acid, by Thermo Fisher Scientific (1 mentions)
Diethylpyrocarbonate depc treated water, by Takara Bio (1 mentions)
Glutathione (gsh), by Merck Group (1 mentions)
Chloroquine diphosphate, by Merck Group (1 mentions)
Miltefosine, by Merck Group (1 mentions)
Doxorubicin, by Merck Group (1 mentions)
Hypoxanthine, by Merck Group (1 mentions)
N acetyl d glucosamine, by Merck Group (1 mentions)
Amphotericin b, by Merck Group (1 mentions)
3 protocols using sybr 1
1
Profiling Gut Bacterial 16S rDNA
2
Synthesis and Activation of Ru(bpy)3 Complexes
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The chemical reagents for the synthesis of Ru(bpy)32+ and activated Ru(bpy)32+, including cis-bis(2,2′-bipyridine) dichlororuthenium (II), 2,2′-bipyridine-4,4′-dicarboxylic acid, N-hydroxysuccinimide (NHS), N,N′-dicyclohexylcarbodiimide (DCC), N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDC), sodium hexafluorophosphate, and sodium borate, were obtained from Alfa Aesar Co., Ltd. Streptavidin magnetic beads were synthesized by New England BioLabs. Diethylpyrocarbonate (DEPC)-treated water and RNase inhibitor were obtained from Takara Biotechnology (Dalian) Co., Ltd. PBS (20× solution) and reagents related to electrophoresis were purchased from Shanghai Sangon Biotechnology Co. Ltd. SYBR I and SYBR II were purchased from Invitrogen. Reagent-grade L-lysine, t-butyloxycarbonyl (Boc), trifluoroacetic acid (TFA), and glutathione (GSH) were purchased from Sigma-Aldrich and used without further purification except where noted. All oligonucleotides and probes synthesized in this work were purified by Invitrogen. The commercial kit for telomerase extraction was obtained from Millipore Co. Ltd.
3
Plasmodium Falciparum Lysis Buffer Protocol
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Chloroquine diphosphate (CQ), primaquine (PQ), doxorubicin, miltefosine, amphotericin B, hypoxanthine and N-acetyl-D-glucosamine were acquired from Sigma ® , Imodocarbe dipropionate (Carbesia ® ), was purchased from MSD Animal Health (France), Diminazene aceturate (Veriben ® ) was acquired from Ceva-Libourne, France and SYBR I was from INVITROGEN ® . hypoxanthine was prepared as a stock solution at 10 mM. SYBR I was prepared as a stock solution in DMSO at 100X concentration which was stored at -20°C protected from light and thawed immediately before use. The lysis buffer consists of a solution of Tris (20 mM), EDTA (5 mM), saponin (0.008% [w/v]) and Triton X-100 (0.08% [v/v]), which was previously prepared, adjusted to pH 7.5 and stored at 4°C.
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