Rest 2009 version 2

Manufactured by Qiagen

The REST 2009 version 2.0.13 is a lab equipment product by Qiagen. It is a software program designed for the analysis of gene expression data. The core function of the REST 2009 version 2.0.13 is to perform relative quantification of gene expression data using the comparative CT method.

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REST 2009 software (128 citations)

2 protocols using rest 2009 version 2

Statistical Analysis of Continuous Data

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Continuous data were analyzed by using unpaired Student's t test except for comparison of real-time PCR data which were made by pairwise fixed reallocation randomization test using REST software (REST 2009 version 2.0.13; Qiagen GmbH; http://www.gene-quantification.de/rest-2009.html) (46 (link)). At least three experiments were repeated and the results were presented as the mean ± standard deviation. Comparison of bisulfite sequencing data was made by Fisher's exact test. P<0.05 was considered to indicate a statistically significant difference.

Moon I.Y, & Kim J.W. (2021). Methylation profile of hepatitis B virus is not influenced by interferon α in human liver cancer cells. Molecular Medicine Reports, 24(4), 715.

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Selecting Stable Housekeeping Genes for Honeybee Expression Analysis

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Four A. mellifera HK genes (GAPDH, EF, β-Actin and 18S; S1 Table) were evaluated to select the most appropriate ones for analysis [22 (link)]. To determine the stability of expression (M) of the selected reference genes in the honeybee tissues studied (ventriculi), the four HK genes were analyzed in duplicate in all the samples and the Ct values were subsequently determined with the geNorm software [23 ]. The two most stable genes (lowest M value) were EF and β-Actin and they were selected as the HK genes for the subsequent analysis of expression.
The relative expression for each gene was calculated using the Relative Expression Software Tools: REST MCS-version 2 (http://www.gene-quantification.de/download.html) and REST 2009-version 2.0.13 (Qiagen GmbH), which use the pair wise fixed reallocation randomization test [24 (link), 25 (link)]. All the groups infected with either N. apis or N. ceranae (irrespective of the treatment with cycloheximide) were compared with the uninfected control groups using this software and the efficiency of every reaction was taken into account as recommended elsewhere [24 (link)]. The level of significance for determining the up or down regulation for each gene was also determined using this software.

Martín-Hernández R., Higes M., Sagastume S., Juarranz Á., Dias-Almeida J., Budge G.E., Meana A, & Boonham N. (2017). Microsporidia infection impacts the host cell's cycle and reduces host cell apoptosis. PLoS ONE, 12(2), e0170183.

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