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Chromium single cell atac reagent kits v1 user guide

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The Chromium Single Cell ATAC Reagent Kits V1 User Guide provides instructions for using the Chromium Single Cell ATAC Solution to profile chromatin accessibility in individual cells. The user guide outlines the necessary steps and reagents required to generate single-cell ATAC-seq libraries from cell samples.

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Chromium Single Cell ATAC Kit (4 citations) Chromium Single Cell ATAC Reagent Kits (3 citations) Chromium Single Cell Reagent Kit (3 citations) Single Cell ATAC Reagent Kits (2 citations)

4 protocols using chromium single cell atac reagent kits v1 user guide

1

Single-Cell ATAC-Seq Using Chromium

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SnATACseq was performed following the Chromium Single Cell ATAC Reagent Kits V1 User Guide (10x Genomics, Pleasanton, CA). Nuclei were counted (Countess counter), transposition was performed in 10 μl at 37 C for 60 min on at least 1,000 nuclei, before loading of the Chromium Chip E (PN-2000121). Barcoding was performed in the emulsion (12 cycles) following the Chromium protocol. Libraries were indexed for multiplexing (Chromium i7 Sample Index N, Set A kit PN-3000262). Same-sex samples were processed together from nuclei isolation to sequencing, with the three female samples processed one day, and the three male samples processed another day.
Ruf-Zamojski F., Zhang Z., Zamojski M., Smith G.R., Mendelev N., Liu H., Nudelman G., Moriwaki M., Pincas H., Castanon R.G., Nair V.D., Seenarine N., Amper M.A., Zhou X., Ongaro L., Toufaily C., Schang G., Nery J.R., Bartlett A., Aldridge A., Jain N., Childs G.V., Troyanskaya O.G., Ecker J.R., Turgeon J.L., Welt C.K., Bernard D.J, & Sealfon S.C. (2021). Single nucleus multi-omics regulatory landscape of the murine pituitary. Nature Communications, 12, 2677.
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2

Single-Cell ATAC-Seq Chromium Protocol

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SnATACseq was performed following the Chromium Single Cell ATAC Reagent Kits V1 User Guide (10x Genomics, Pleasanton, CA). Nuclei were counted (Countess counter), transposition was performed in 10 μL at 37C for 60 min on at least 1,000 targeted nuclei, before loading of the Chromium Chip E (PN-2000121). Barcoding was performed in the emulsion (12 cycles) following the Chromium protocol. Libraries were indexed for multiplexing (Chromium i7 Sample Index N, Set A kit PN-3000262).
Zhang Z., Zamojski M., Smith G.R., Willis T.L., Yianni V., Mendelev N., Pincas H., Seenarine N., Amper M.A., Vasoya M., Cheng W.S., Zaslavsky E., Nair V.D., Turgeon J.L., Bernard D.J., Troyanskaya O.G., Andoniadou C.L., Sealfon S.C, & Ruf-Zamojski F. (2022). Single nucleus transcriptome and chromatin accessibility of postmortem human pituitaries reveal diverse stem cell regulatory mechanisms. Cell reports, 38(10), 110467.
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3

Single-Cell ATAC-Seq Chromium Protocol

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SnATACseq was performed following the Chromium Single Cell ATAC Reagent Kits V1 User Guide (10x Genomics, Pleasanton, CA). Nuclei were counted (Countess counter), transposition was performed in 10 μL at 37C for 60 min on at least 1,000 targeted nuclei, before loading of the Chromium Chip E (PN-2000121). Barcoding was performed in the emulsion (12 cycles) following the Chromium protocol. Libraries were indexed for multiplexing (Chromium i7 Sample Index N, Set A kit PN-3000262).
Zhang Z., Zamojski M., Smith G.R., Willis T.L., Yianni V., Mendelev N., Pincas H., Seenarine N., Amper M.A., Vasoya M., Cheng W.S., Zaslavsky E., Nair V.D., Turgeon J.L., Bernard D.J., Troyanskaya O.G., Andoniadou C.L., Sealfon S.C, & Ruf-Zamojski F. (2022). Single nucleus transcriptome and chromatin accessibility of postmortem human pituitaries reveal diverse stem cell regulatory mechanisms. Cell reports, 38(10), 110467.
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4

Chromium Single Cell ATAC-seq Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
SnATACseq was performed following the Chromium Single Cell ATAC Reagent Kits V1 User Guide (10x Genomics, Pleasanton, CA). Nuclei were counted (Countess counter), transposition was performed in 10 μl at 37C for 60min on at least 1,000 targeted nuclei, before loading of the Chromium Chip E (PN-2000121). Barcoding was performed in the emulsion ( 12cycles) following the Chromium protocol. Libraries were indexed for multiplexing (Chromium i7 Sample Index N, Set A kit PN-3000262).
Zhang Z., Zamojski M., Smith G.R., Willis T.L., Yianni V., Mendelev N., Pinças H., Seenarine N., Amper M.A.S., Vasoya M., Nair V.D., Turgeon J.L., Bernard D.J., Troyanskaya O.G., Andoniadou C.L., Sealfon S.C., & Ruf-Zamojski F. (2021). Single nucleus pituitary transcriptomic and epigenetic landscape reveals human stem cell heterogeneity with diverse regulatory mechanisms.
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