Anti rabbit phospho nf κb p65 rabbit antibody

For cell culture and reagents, primary mouse pulmonary microvascular endothelial cells were obtained from Angio-Proteomie (Boston, MA, US) and maintained in Angio-Proteomie Endothelial Cell Medium in a humidified 37°C, 5% CO₂ incubator. The medium was changed at 48-hour intervals. Genistein-3′-sodium sulfonate (GSS) was obtained from Shanghai Tianxi Chemical Co., Ltd. (Shanghai, China), and BAY11-7082 was purchased from Beyotime of China. The LPS (from Escherichia coli 055:B5) was obtained from Sigma-Aldrich (St. Louis, MO). The anti-GEF-H1 rabbit monoclonal antibody (mAb), anti-Myd88 rabbit mAb, anti-NF-κB p65 rabbit mAb, and anti-rabbit phospho-NF-κB-p65 rabbit antibody were obtained from Cell Signaling Technologies (Danvers, MA). Alexa Fluor 594-conjugated goat anti-rabbit secondary antibody and the ProLong Gold Antifade Mountant with DAPI were obtained from Invitrogen Life Science.

Primary mouse pulmonary micro‐vascular endothelial cells (MPMECs) were obtained Angio‐Proteomie and maintained in Angio‐Proteomie Endothelial Cell Medium in a humidified 37°C, 5% CO₂ incubator. GSS was obtained from Shanghai Tian Xi Chemical Co., Ltd., and BAY11‐7082 was purchased from Beyotime of China. The LPS was obtained from Sigma‐Aldrich. Anti‐GEF‐H1 rabbit monoclonal antibody (mAb), anti‐Myd88 rabbit mAb, anti‐NF‐κB p65 rabbit mAb, anti‐rabbit phospho‐NF‐κB‐p65 rabbit antibody, anti‐BCL‐2 rabbit mAb and anti‐PARP rabbit mAb were obtained from Cell Signaling Technology. Anti‐CD31 antibody and anti‐cleaved‐PARP antibody were purchased from Santa Cruz Biotechnology. The Alexa Fluor 555‐conjugated goat antimouse secondary antibody and Alexa Fluor 594‐conjugated goat anti‐rabbit secondary antibody and the ProLong Gold Antifade Mountant with DAPI were obtained from Invitrogen Life Sciences.