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2 protocols using norfloxacin

1

Fluoroquinolone and Dicarboxylic Acid Protocol

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Ciprofloxacin,
norfloxacin, enrofloxacin,
suberic acid, azeliac acid, and sebacic acid were obtained from Tokyo
Chemical Industry (TCI, Europe). Glutaric acid, adipic acid, and pimelic
acid were obtained from Sigma-Aldrich. The solvents methanol, acetonitrile
(Merck Millipore), ethyl acetate (Sigma-Aldrich), acetone (Fisher
Scientific), ethanol (Fisher Scientific), propylene glycol (TCI, Europe),
and polyethylene glycol 400 (Sigma-Aldrich) were analytical grade
and used as received.
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2

Fluoroquinolone Antioxidant Evaluation in Cells

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5, 5-dimethyl-1-pyrroline-N-oxide (DMPO) was purchased from Alexis Biochemicals (Lausen, Switzerland). Ciprofloxacin (CPFX), norfloxacin (NRFX), pazfloxacin (PZFX), levofloxacin (LVFX), lomefloxacin (LFLX) and 1, 3-dimethylthiourea (DMTU) were purchased from Tokyo Chemical Industry Co., Ltd (Tokyo, Japan). Diethylenetriaminepentaacetic acid (DTPA), NaNO2, NaNO3 and dextran were purchased from Nacalai Tesque (Kyoto, Japan). Ficoll-Paque was purchased from GE Healthcare (Tokyo, Japan), and phorbol 12-myristate 13-acetate (PMA) was purchased from Wako Pure Chemical Industries, Ltd (Osaka, Japan). Mayer’s hematoxylin and eosin alcohol solutions were purchased from Muto Pure Chemicals (Tokyo, Japan). Superoxide dismutase from bovine erythrocytes was purchased from Sigma (Tokyo, Japan).
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