Anti phospho erk1 2 rabbit monoclonal antibody

Western blot was performed as previously reported [40 (link)]. Primary antibodies were anti-ABHD2 rabbit polyclonal antibody (1:250, Abgent AP13083c, San Diego, USA), anti-phospho ERK1/2 rabbit monoclonal antibody (1:1,000, Cell Signal Technology, 197G2, Danver, JAPAN), anti-ERK1/2 rabbit monoclonal antibody (1:1000, Cell Signal Technology, 137F5), anti-phospho p38 MAPK rabbit monoclonal antibody (1:1000, Cell Signal Technology, (D13E1)XP) anti-p38MAPK rabbit monoclonal antibody (1:1000, Cell Signal Technology, (D3F9)XP) and anti-GAPDH mouse monoclonal antibody (1:1000, Abcam, ab8245, Cambridge, UK). After washing in tris-buffered saline (TBS)-T, the blots were incubated with the appropriate peroxidase-coupled secondary antibody (1:2000; Anti-rabbit HRP or anti-mouse HRP, GE Healthcare Life Science). Specific proteins were detected using ECL Plus Western Blotting Reagent (GE Healthcare Life Science). The bands were visualized using Molecular Imager Gel DocTMXR+ and ChemiDocTMXRS+ Systems with Image Lab 2.0 software (Bio-Rad).

Western blotting (WB) was performed as previously described [11 (link)]. The primary antibodies employed were anti-DAB2 rabbit monoclonal antibody (dilution 1/500), anti-E-cadherin mouse monoclonal antibody (cat no. 3195; dilution 1/500, Cell Signaling Technology, Danvers, MA, USA), anti-N-cadherin mouse monoclonal antibody (cat no. 33-3900; dilution 1/500, Thermo Fisher Scientific), anti-vimentin rabbit monoclonal antibody (cat no. 5741; dilution 1/500, Cell Signaling Technology), anti-phospho-AKT rabbit monoclonal antibody (cat no. 4060; dilution 1/1000, Cell Signaling Technology), anti-phospho-ERK1/2 rabbit monoclonal antibody (cat no. 9101; dilution 1/1000, Cell Signaling Technology). Anti-actin mouse monoclonal antibody (clone AC-15; dilution 1/10,000, Sigma-Aldrich, Tokyo, Japan) was used as an inner loading control. Secondary antibody, horseradish peroxidase-conjugated goat anti-rabbit IgG (dilution 1/5000) or anti-mouse IgG antibody (dilution 1/10,000) (Santa Cruz Biotechnology), was incubated at 37 °C for 1 h.

To examine CD44v6 and CD44 protein levels, cell lysates were subjected to Western blotting analyses using an anti-human CD44v6 mouse monoclonal antibody (clone no. VFF-7; Santa Cruz Biotechnology) and anti-CD44 mouse monoclonal antibody (clone no. DF1485, 1:1000 dilution; Santa Cruz Biotechnology). A control, glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was examined using an anti-GAPDH antibody (clone no. G-9; Santa Cruz Biotechnology).
To examine c-Met and Erk phosphorylation, MDA-MB231 cells were serum-starved for 24 h, pre-treated with CD44v6-binding peptides (20 µM) for 10 min, and incubated with 25 ng/mL HGF for 10 min. After the incubation, the cell lysates were subjected to Western blotting analyses using an anti-phospho-Met rabbit monoclonal antibody (clone no. D26; Cell Signaling Technology), anti-Met mouse monoclonal antibody (clone no. 25H2, 1:1000 dilution; Cell Signaling Technology), anti-phospho-Erk1/2 rabbit monoclonal antibody (clone no. 197G2; Cell Signaling Technology), and anti-ERK1 rabbit polyclonal antibody (1:1000 dilution; Santa Cruz Biotechnology). The labeled bands on immunoblots were detected using enhanced chemiluminescence reagents (ThermoFisher Scientific).