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Protectin dx

Manufactured by Cayman Chemical
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Sourced in United States

Protectin DX is a laboratory reagent produced by Cayman Chemical. It is a chemical compound used in research applications.

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Lab products found in correlation

Lps escherichia coli serotype 055 b5, by Merck Group (1 mentions) Camp elisa kit, by R&D Systems (1 mentions) Nedd4 2, by Cell Signaling Technology (1 mentions) Ly294002, by Cayman Chemical (1 mentions) Ns 398, by Cayman Chemical (1 mentions) Mk0524, by Cayman Chemical (1 mentions) 15 r 15 methyl pgd2, by Cayman Chemical (1 mentions) Bw245c, by Cayman Chemical (1 mentions) Cay10471, by Cayman Chemical (1 mentions) At 56, by Cayman Chemical (1 mentions) 17r hdha, by Cayman Chemical (1 mentions)

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Protectins (2 citations)

4 protocols using protectin dx

1

Protectin DX Modulates Inflammatory Signaling

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Protectin DX, LY294002 (PI3K inhibitor), and H89 (PKA inhibitor) were from Cayman Chemical Company (Ann Arbor, MI). LPS (Escherichia coli serotype 055:B5) was purchased from Sigma (St. Louis, MO). Interleukin-1 (IL-1), interleukin-10 (IL-10), tumor necrosis factor-α, myeloperoxidase, and cAMP ELISA kits were from R&D Systems (Minneapolis, MN). BOC-2 (ALX inhibitor), Rp-cAMP (cAMP inhibitor), and Rp-cGMP (cGMP inhibitor) were obtained from Biomol-Enzo Life Sciences (Farmingdale, NY). Anti-Na, K-ATPase α1 and β1 antibodies were purchased from Abcam (Cambridge, MA), and anti-sodium channel α, β, and γ antibodies were purchased from Biorbyt (Cambridge, Cambridgeshire). Anti-P-Akt, total Akt (T-Akt), and Nedd4–2 antibodies were obtained from Cell Signaling Technology (Beverly, MA).
Zhuo X.J., Hao Y., Cao F., Yan S.F., Li H., Wang Q., Cheng B.H., Ying B.Y., Smith F.G, & Jin S.W. (2018). Protectin DX increases alveolar fluid clearance in rats with lipopolysaccharide-induced acute lung injury. Experimental & Molecular Medicine, 50(4), 49.
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2

Pharmacological Modulation of Lipid Mediators

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Protectin DX, NS-398(selective COX-2 inhibitor), AT-56 (L-PGDS inhibitor), BW245C (DP1 receptor agonist), 15(R)-15-methyl-PGD2(CRTH2/DP2 receptor agonist), MK-0524(DP1 receptor antagonist) and CAY-10471(CRTH2/DP2 receptor antagonist) were obtained from Cayman Chemical (Ann Arbor, MI, USA), Lipopolysaccharide (Escherichia coli O55: B5), BOC-2 (ALX/FPR2 receptor inhibitor) were purchased from Biomol/Enzo Life Sciences (Farmingdale, NY, USA).
Hu X., Zhang Y.A., Chen B., Jin Z., Lin M.L., Li M., Mei H.X., Lu J.C., Gong Y.Q., Jin S.W, & Zheng S.X. (2021). Protectin DX promotes the inflammatory resolution via activating COX-2/L-PGDS-PGD2 and DP1 receptor in acute respiratory distress syndrome. International Immunopharmacology, 102, 108348.
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3

Protectin DX Preparation and Inhibitor Use

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Protectin DX was purchased from Cayman Chemical. The chemical structure of PDX was shown in Figure 1D. PDX was dissolved in ethanol as supplied by the manufacturer and was stored at −80°C. Before use, ethanol was evaporated under a gentle stream of nitrogen; then, PDX was dissolved immediately in sterile saline or culture medium to the desired concentrations. Inhibitors were used at the following concentrations according to manufacturer's instructions: LY294002 (a PI3‐kinase inhibitor) at 10 µmol/L, and BOC‐2 (N‐t‐Boc‐Phe‐Leu‐Phe‐Leu‐Phe, ALXR antagonist) at 10 µmol/L. Cells were incubated with inhibitors for 1 h prior to every treatment. Appropriate vehicle controls were used for all experiments with inhibitors.
Yang J., Li M., Hu X., Lu J., Wang Q., Lu S., Gao F., Jin S, & Zheng S. (2020). Protectin DX promotes epithelial injury repair and inhibits fibroproliferation partly via ALX/PI3K signalling pathway. Journal of Cellular and Molecular Medicine, 24(23), 14001-14012.
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4

SPM Precursors and SPMs Administration

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SPM precursors and SPMs were injected as previously described at a concentration that leads to elevated levels of 14-HDHA, 17-HDHA, and PDX.43 (link) Briefly, 14(S)-HDHA (0.1 mg/ml), 17R-HDHA (0.1 mg/ml), and Protectin DX (0.1 mg/ml) were purchased from Cayman Chemicals (Ann Arbor, MI). SPMs were prepared in ethanol in the dark and kept on ice at all times. The final cocktail concentration was 900 ng per mouse (300 ng of each SPM precursor and SPM) in PBS or vehicle control (ethanol in PBS) and was administered i.p. to lean and obese mice for 4 consecutive days followed by euthanasia on day 5. Control mice were administered a vehicle control.
Brito B., Martínez M., Fernández D., Rey L., Cabrera E., Palacios J.M., Imperial J, & Ruiz-Argüeso T. (1997). Hydrogenase genes from Rhizobium leguminosarum bv. viciae are controlled by the nitrogen fixation regulatory protein NifA. Proceedings of the National Academy of Sciences of the United States of America, 94(12), 6019-6024.
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