In this study, Milli-Q water (18.2 MΩ) was used to prepare all aqueous solutions. Chloroauric(III) acid tetrahydrate (HAuCl4, Nacalai Tesque, Kyoto, Japan), trisodium citrate dihydrate (Kanto Chemical, Tokyo, Japan), hydrochloric acid (Kishida Chemical, Osaka, Japan), silver nitrate (Fujifilm Wako Pure Chemical, Osaka, Japan), L-ascorbic acid (Tokyo Chemical Industry, Tokyo, Japan), 1-butanol (BuOH, Kishida Chemical), ethanol (Kishida Chemical), sodium hydroxide (Kanto Chemical), 16-mercaptohexadecanoic acid (MHA, Sigma–Aldrich, St. Louis, MO, USA), polyethyleneimine (PEI, MW: ~10,000, Fujifilm Wako Pure Chemical) and PVP (MW: ~55,000, Sigma–Aldrich, Saint Louis, MO, USA) were used without further purification.
Polyethyleneimine pei
Manufactured by Fujifilm
Sourced in United States, Japan
Polyethyleneimine (PEI) is a versatile polymer that can be used in various laboratory applications. It is a water-soluble, cationic polymer with a high charge density, making it useful for applications such as nucleic acid or protein purification, cell transfection, and as a coating agent. PEI is available in different molecular weights and configurations to meet the specific requirements of different laboratory protocols.
Automatically generated - may contain errors
Lab products found in correlation
Sodium hydroxide (naoh), by Kanto Chemical (1 mentions)
Silver nitrate, by Fujifilm (1 mentions)
Trisodium citrate dihydrate, by Kanto Chemical (1 mentions)
L ascorbic acid, by Tokyo Chemical Industry (1 mentions)
16 mercaptohexadecanoic acid mha, by Merck Group (1 mentions)
Trypsin, by Merck Group (1 mentions)
Polyvinyl alcohol (pva), by Merck Group (1 mentions)
Toluene, by Merck Group (1 mentions)
N dodecane, by Merck Group (1 mentions)
Dichloromethane dcm, by Merck Group (1 mentions)
Mfs rfd240na, by Advantec (1 mentions)
Methanol, by Kanto Chemical (1 mentions)
4 protocols using polyethyleneimine pei
1
Synthesis and Characterization of Gold Nanoparticles
2
Isolation and Differentiation of Rat eNSCs
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All eNSCs were obtained from telencephalon tissues of E14 Wistar Wt and Tg [Wistar-esTgN(Sox2/CD63-GFP)3NCCRI] rats. Isolated eNSCs were cultured on noncoated tissue culture plastic as neurospheres in KBM neural stem cell proliferation medium (Kohjin Bio, Saitama, Japan) with 0.2% KBM supplement containing EGF and bFGF (Kohjin Bio) at 37°C in air enriched with 5% CO2, as described previously (Yoshimura et al., 2016a (link)). After 3 or 4 days, neurospheres were dissociated using trypsin (Sigma-Aldrich) and passaged. Differentiation was carried out by seeding on tissue culture dishes pre-coated with 0.2% polyethyleneimine (PEI) (Wako). Differentiation medium consisted of KBM containing 2% B-27 serum-free supplement containing vitamin A (Life Technologies) without EGF and bFGF. Dissociated cells were plated at a density of 1.8×106 cells per 35 mm culture dish (Becton Dickinson). For immunocytochemistry, 1.4×105 cells/0.2 ml were reseeded on pre-coated glass-bottomed dishes (Matsunami, Osaka, Japan). The culture medium was changed 3 or 4 days after plating, and differentiation proceeded for 7 days. To promote the proliferation of differentiated astroglial cells, culture medium was replaced with astroglial culture medium (described in the next section) at 6 days after differentiation.
3
Preparation of PDA Particles and Emulsions
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Reagents used to prepare PDA particles were dopamine hydrochloride (DA–HCl, Kanto Chemical, Tokyo, Japan), tris(hydroxymethyl)aminomethane (Tris, Kanto Chemical), and methanol (Kanto Chemical). Oils used to prepare emulsions were n-dodecane (≥99%, Sigma-Aldrich, Tokyo, Japan), toluene (99%, Sigma-Aldrich), dichloromethane (DCM, ≥99.0%, Sigma-Aldrich), methyl myristate (95.0%, Wako Pure Chemical, Osaka, Japan), octafluorotoluene (97%, Wako Pure Chemical) and perfluorononane (99%, Wako Pure Chemical). Poly(ethylene imine) (PEI, Average Molecular Weight, approximately 600, Wako Pure Chemical) was used as a crosslinking agent for PDA particles. Poly(vinyl alcohol) (PVA) were purchased from Sigma-Aldrich. Deionized water (<0.06 µS·cm−1, Advantec MFS RFD240NA: GA25A-0715) was used for preparation of PDA particles and emulsions. All other chemicals and solvents were of reagent grade and used as received.
4
Enzymatic Alcohol Oxidation Assay
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Alcohol oxidase (AOD, A2404-1KU, from Pichia pastoris) was obtained from Sigma-Aldrich Japan (Tokyo, Japan). Formaldehyde dehydrogenase (FALDH, from Pseudomonas sp.) was from two different manufacturers, Funakoshi (Tokyo, Japan) and Toyobo (Osaka, Japan). An oxidized form of β-nicotinamide adenine dinucleotide (NAD+) was purchased from Oriental Yeast (Tokyo, Japan). A hydrophilic polytetrafluoroethylene (H-PTFE) membrane (thickness: 80 μm, porosity: 80%, pore size: 0.2 μm) was from Millipore (Burlington, MA, USA). Poly [2-methacryloyloxyethyl phosphorylcholine (MPC)-co-2-ethylhexyl methacrylate (EHMA)] (PMEH) was synthesized in house by the free radical-polymerization method [42 (link)]. Polyethyleneimine (PEI, average molecular weight of 10,000, 164-17821) and glutaraldehyde (GA, 079-00533) were purchased from Fujifilm Wako Pure Chemical (Osaka, Japan).
All reagents for the following buffer solutions were obtained from Fujifilm Wako Pure Chemical (Osaka, Japan). For the phosphate buffer (PB, 100 mM) solution, we added potassium dihydrogen phosphate solution (100 mM in ultrapure water) to disodium hydrogen phosphate solution (100 mM in ultrapure water) to buffer the solution pH from 6.5 to 8.5. For the Tris-HCl solution (100 mM), we added HCl solution to 100 mM trimethylolaminomethane solution to buffer the pH from 8.5 to 10.5.
All reagents for the following buffer solutions were obtained from Fujifilm Wako Pure Chemical (Osaka, Japan). For the phosphate buffer (PB, 100 mM) solution, we added potassium dihydrogen phosphate solution (100 mM in ultrapure water) to disodium hydrogen phosphate solution (100 mM in ultrapure water) to buffer the solution pH from 6.5 to 8.5. For the Tris-HCl solution (100 mM), we added HCl solution to 100 mM trimethylolaminomethane solution to buffer the pH from 8.5 to 10.5.
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