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Biaevalution

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The BIAevaluation is a label-free, real-time biomolecular interaction analysis system developed by GE Healthcare. It is designed to measure the kinetics and affinity of molecular interactions between a wide range of biomolecules, including proteins, peptides, small molecules, and nucleic acids. The system utilizes surface plasmon resonance (SPR) technology to detect and quantify these interactions in a highly sensitive and accurate manner.

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Lab products found in correlation

Cm5 chip, by GE Healthcare (3 mentions) Biacore t200, by GE Healthcare (2 mentions) Biacore t200 instrument, by GE Healthcare (1 mentions) Biacore cm5 sensor chip, by GE Healthcare (1 mentions) Cyclic adp ribose, by Merck Group (1 mentions)

Close spelling variants of this product

BIAevalution software (3 citations) BIAevalution software v4.1 (2 citations)

4 protocols using biaevalution

1

SPR Binding Assay for MDM2-UbcH5B-Ub Interaction

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SPR binding experiments were performed at 25 °C on a Biacore T200 instrument using a CM-5 chip (GE Healthcare) with coupled anti-GST antibody as described previously.20 (link) Briefly, GST-tagged MDM2 variants were coupled on the chip and a serial dilution of UbcH5B–Ub in running buffer containing 25 mM Tris-HCl, pH 7.6, 150 mM NaCl, 1 mM DTT and 0.005% (v/v) Tween-20 was used as analyte. Two technical replicates were performed and data were analyzed with BIAevalution (GE Healthcare) and Scrubber2 (BioLogic Software).
Magnussen H.M, & Huang D.T. (2021). Identification of a Catalytic Active but Non-Aggregating MDM2 RING Domain Variant. Journal of Molecular Biology, 433(5), 166807.
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2

Quantitative Binding Kinetics of UBE2K

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SPR binding experiments were performed at 25 °C on a Biacore T200 instrument using a CM-5 chip (GE Healthcare) with coupled anti-GST antibody as described previously48 (link)
. GST-tagged UBE2K variants were coupled on the chip and a serial dilution of Ub in running buffer containing 25 mM Tris-HCl, pH 7.6, 150 mM NaCl, 1 mM DTT and 0.005% (v/v) Tween-20 was used as analyte. Two technical replicates were performed and data were analyzed with BIAevalution (GE Healthcare) and fit to a one-site binding model using Prism (GraphPad).
Nakasone M.A., Majorek K.A., Gabrielsen M., Sibbet G.J., Smith B.O, & Huang D.T. (2022). Structure of UBE2K–Ub/E3/polyUb reveals mechanisms of K48-linked Ub chain extension. Nature Chemical Biology, 18(4), 422-431.
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3

SPR Binding Assay for UBE2K

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SPR binding experiments were performed at 25 °C on a Biacore T200 instrument using a CM-5 chip (GE Healthcare) with coupled anti-GST antibody as described previously48 (link). GST-tagged UBE2K variants were coupled on the chip and a serial dilution of Ub in running buffer containing 25 mM Tris–HCl, pH 7.6, 150 mM NaCl, 1 mM DTT and 0.005% (v/v) Tween-20 was used as analyte. Two technical replicates were performed and data were analyzed with BIAevalution (GE Healthcare) and fit to a one-site binding model using Prism (GraphPad).
Nakasone M.A., Majorek K.A., Gabrielsen M., Sibbet G.J., Smith B.O, & Huang D.T. (2022). Structure of UBE2K–Ub/E3/polyUb reveals mechanisms of K48-linked Ub chain extension. Nature Chemical Biology, 18(4), 422-431.
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4

Biacore Analysis of ADP-ribose Binding

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Recombinant RCD1-His and GST-RCD1ΔWWE proteins were coupled to a Biacore CM5 sensor chip (GE Healthcare) via amino-groups. PAR (625 nM) (Trevigen) was profiled at a flow rate of 30 mL/min for 300 s, followed by 600 s flow of wash buffer (10 mM HEPES, pH 7.4, 150 mM NaCl, 3 mM EDTA, 0.05% Surfactant P20). Mono ADP-ribose (Sigma) and cyclic ADP-ribose (Sigma) were profiled at 1 mM concentration. After analysis in BiaEvalution (Biacore, GE Healthcare), the normalized resonance units were plotted over time with the assumption of one-to-one binding.
Vainonen J.P., Gossens R., Krasensky-Wrzaczek J., De Masi R., Danciu I., Puukko T., Battchikova N., Jonak C., Wirthmueller L., Wrzaczek M., Shapiguzov A, & Kangasjärvi J. (2023). Poly(ADP-ribose)-binding protein RCD1 is a plant PARylation reader regulated by Photoregulatory Protein Kinases. Communications Biology, 6, 429.
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