Mouse monoclonal anti myot ab b 3

Culture media (RPMI-1640), fetal bovine serum, antibiotic–antimycotic solution, L-glutamine, DAPI mounting medium, and PAC and TOP were purchased from Sigma (Sigma-Aldrich, Poznan, Poland). Rabbit monoclonal anti-ALDH1A1 Ab was purchased from Abcam (Abcam, Cambridge, UK), mouse monoclonal anti-COL3A1 was purchased from Invitrogen (Invitrogen, Carlsbad, CA, USA), mouse monoclonal anti-PTPRK Ab, anti-p-Tyr, mouse monoclonal anti-MYOT Ab (B-3), and rabbit polyclonal anti-GADPH Ab were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Mouse monoclonal anti-P-gp Ab was purchased from Invitrogen (Thermo Fisher Scientific, Waltham, MA, USA). Rabbit polyclonal anti-TGFBI was purchased from Atlas Antibodies (Stockholm, Sweden). Mouse anti-Ki67 was purchased from Dako (Glostrup, Denmark). Donkey anti-goat horseradish peroxidase (HRP)-conjugated Ab was purchased from Santa Cruz Biotechnology (Santa Cruz Biotechnology Inc., Dallas, TX, USA). The fluorescent MFP488 donkey anti-goat IgG was obtained from MoBiTec (MoBiTec, Molecular Biotechnology, Goettingen, Germany) and fluorescent Alexa Fluor^®488 and Alexa Fluor^®594 Donkey Anti-Rabbit IgG from Jackson ImmunoResearch Laboratories (Jackson ImmunoResearch Laboratories, Cambridgeshire, UK). Western blot reagents (membranes, gels and protein marker) were purchased from Bio-Rad (Bio-Rad Laboratories Ltd., Watford, Hertfirdshire, UK).