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Nis elements ar v3.22 software

Manufactured by Nikon

NIS‐Elements AR (v3.22) is Nikon's image analysis software. It provides a platform for acquisition, processing, and analysis of digital microscope images.

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2 protocols using nis elements ar v3.22 software

1

Epifluorescence microscopy imaging protocol

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Epifluorescence microscopy images were obtained with an Eclipse Ti‐ microscope (Nikon) controlled by NIS‐Elements AR (v3.22) software (Nikon) equipped with an iXon+897 EMCCD camera (Andor). For each channel, all camera and microscope settings were kept constant across all samples.
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2

Cell Motion Tracking via Phase-Contrast Microscopy

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Recording of cell motion using phase‐contrast video microscopy was done on an Eclipse Ti microscope (Nikon) controlled by NIS‐Elements AR (v3.22) software (Nikon). Detection was implemented using an iXon+897 EMCCD camera (Andor) as 14‐bit images. Atmosphere control was achieved using INUB‐GSI stage top incubator (Tokai Hit) set to 37°C and a GM‐4000 Gas Mixer (Tokai Hit) set to 5% CO2 and 160 ml/min. For every replicate, recording was set over a period of 120 s at a frame rate of 25 frames per second.
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