Versatrek redox 1

Blood samples were inoculated into VersaTrek Redox 1 aerobic and Redox 2 anaerobic media (Thermo Fisher Scientific, Waltham, MA) and evaluated using the VersaTREKTM automated microbial detection system (TREK Diagnostic Systems, Cleveland, OH, USA). Incubation was continued for up to 5 days or until a positive culture was observed. Positive bacterial cultures were examined for pathogen type and antibiotic sensitivity. VITEK 2^® COMPACT (bioMérieux, Marcy-I'Étoile, France) was used to identify bacteria and their antibiotic sensitivity. GN and GP cards were used to identify gram-negative and gram-positive microorganisms, respectively. AST-GN204 and AST-GN222 labels were used to test antibiotic sensitivity in gram-negative bacteria. AST-GP67 and AST-GP 03 cards were used to determine gram-positive sensitivity. The VITEK 2^® COMPACT was used to determine multiresistance phenotypically from the antibiotic susceptibility profile. The Clinical Laboratory Standards Institute standard was used to interpret drug susceptibility data (CLSI, United States). MDROs were defined as those resistant to at least one agent in three or more antimicrobial categories.

Blood specimens were inoculated into a VersaTrek Redox 1 aerobic and Redox 2 anaerobic media (Thermo Fisher Scientific, Waltham, MA) and analyzed by the automated microbial detection system (VersaTREK™, TREK Diagnostic Systems, Cleveland, OH, USA). Incubation was continued until a positive culture was observed or up to a maximum of 5 days. Positive bacterial cultures were tested for the type of pathogen and sensitivity to antibiotics. The identification of bacteria and antibiotic sensitivity was performed using VITEK 2® COMPACT (bioMérieux, Marcy-I'Étoile, France). Gram-negative and Gram-positive bacteria were identified using GN and GP cards, respectively. Antibiotic sensitivity of Gram-negative bacteria was performed using AST-GN204 and AST-GN222 labels. The sensitivity of Gram-positive was determined using AST-GP67 and AST-GP 03 cards. While Candida species were identified using the VITEK2 YST ID card and their susceptibility was determined using the VITEK2 fungal susceptibility card AST-YS-08. Multiresistance was determined phenotypically from the antibiotic susceptibility profile using the VITEK 2® COMPACT. Interpretations of drug susceptibility data were based on the Clinical Laboratory Standards Institute standard (CLSI, United States).