Cd115 clone afs98

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CD115 (clone AFS98) is a mouse monoclonal antibody that recognizes the CD115 antigen. CD115, also known as colony-stimulating factor 1 receptor (CSF1R), is a transmembrane protein that serves as the receptor for macrophage colony-stimulating factor (M-CSF).

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CD115 (AFS98, (6 citations) AFS98 (5 citations) Clone AFS98 (2 citations) Anti-CD115-biotin (clone AFS98 (2 citations) BV-421–conjugated anti–mouse CD115 (clone AFS98, (2 citations)

3 protocols using cd115 clone afs98

Immune Cell Characterization by Flow Cytometry

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Cells were stained with CD45.2 (clone 104), CD19 (clone 6D5), CD3 (clone 17A2), CD11b (clone M1/70), CD115 (clone AFS98), Ly-6G (clone 1A8), and Ly-6C (clone HK1.4) (all BioLegend). Leukocytes were identified as CD45 ^high. Myeloid cells were identified as CD45 ^high CD19 ^low CD3 ^low CD11b ^high. Neutrophils were identified as CD45 ^high CD19 ^low CD3 ^low CD11b ^high CD115 ^low Ly-6G ^high. Inflammatory monocytes were identified as CD45 ^high CD19 ^low CD3 ^low CD11b ^high Ly-6G ^low CD115 ^high Ly-6C ^high. B-Lymphocytes were identified as CD45 ^high CD19 ^high CD3 ^low.

Seung H., Wrobel J., Wadle C., Bühler T., Chiang D., Rettkowski J., Cabezas-Wallscheid N., Hechler B., Stachon P., Maier A., Weber C., Wolf D., Duerschmied D., Idzko M., Bode C., von zur Mühlen C., Hilgendorf I, & Heidt T. (2022). P2Y12-dependent activation of hematopoietic stem and progenitor cells promotes emergency hematopoiesis after myocardial infarction. Basic Research in Cardiology, 117(1), 16.

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Flow Cytometry Analysis of Immune Cell Subsets

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The following fluorochrome-conjugated antibodies were used for flow cytometric analysis and FACS sorting: CD34 (clone RAM34), FcgR (CD16 and CD32; clone Ab93); Sca-1 (clone 108113), c-Kit (CD117; clone 2B8), Ly6C (clone HK1.4), Ly6G (clone 1A8), F4/80 (clone BM8), CD115 (clone AFS98), CD317 (clone 927), CD11c (clone N418), CD86 (clone GL-1), B220 (clone RA3–6B2), I-Ab (clone AF6–120.1), CD8a (clone 53–6.7), CD45.1 (clone A20), CD45.2 (clone 104) from BioLegend; CD43 (clone S7), CD11b (clone M1/70), CD135 (clone A2F10.1) from BD Biosciences. Where possible, non-specific antibody binding was prevented by prior incubation with Fc block (anti-CD16 and anti-CD32). Fc blocking was not possible for identification of progenitors by FcgR expression, so cells were stained for FcgR prior to staining with other antibodies. An LSRFortessa instrument (BD Biosciences) was used for flow cytometry and data were analyzed with FlowJo.

Schnabel E.L, & Jones A.L. (2001). Isolation and Characterization of Five Erwinia amylovora Bacteriophages and Assessment of Phage Resistance in Strains of Erwinia amylovora. Applied and Environmental Microbiology, 67(1), 59-64.

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Comprehensive Immune Cell Profiling by Flow Cytometry

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The following anti-mouse antibodies were used (dilutions are indicated): CD45 (clone 30-F11, 1:100), CD11b (clone M1/70, 1:300), Ly6C (clone HK1.4, 1:300), CD115 (clone AFS98, 1:100), CD64 (clone X54-5/7.1, 1:50), Ly6G (clone 1A8, 1:100), and Tim4 (clone RMT4-54, 1:50), MHCII (clone M4-114.15.2, 1:200), CX₃CR1 (clone SA011F11, 1:100), CD14 (clone M5E2, 1:100), CD16 (clone 3G8, 1:100) all purchased from BioLegend, San Diego, CA, USA. Anti-mouse F4/80 (clone A3-1, 1:50) was purchased from BIORAD. Cells were analyzed with BD FACSCanto™ II (BD Bioscience). Flow cytometry analysis was performed using FlowJo software (TreeStar, Ashland, OR, United States).

Mouhadeb O., Ben Shlomo S., Cohen K., Farkash I., Gruber S., Maharshak N., Halpern Z., Burstein E., Gluck N, & Varol C. (2018). Impaired COMMD10-Mediated Regulation of Ly6Chi Monocyte-Driven Inflammation Disrupts Gut Barrier Function. Frontiers in Immunology, 9, 2623.

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