Cells were fixed in 4% PFA. After permeabilization in 0.5% Triton X-100, cells were blocked with 1% BSA and incubated overnight at 4 °C with the following primary antibodies: goat anti-Epcam antibody (R&D Systems), mouse anti-CK19 (Dako) or rabbit anti-Ki67 (Abcam). Secondary antibodies were then incubated for 1 h at room temperature (Life Technologies), and Prolong Gold mounting media with DAPI (Life Technologies) was used for mounting on a glass slide.
Mouse anti ck19
Manufactured by Agilent Technologies
Mouse anti-CK19 is a primary antibody used for the detection of cytokeratin 19 (CK19) in various samples. CK19 is a type I cytokeratin that is commonly expressed in epithelial cells. This antibody can be used in techniques such as immunohistochemistry and Western blotting to identify and quantify CK19-positive cells.
Automatically generated - may contain errors
Lab products found in correlation
Celltracker green cmfda, by Thermo Fisher Scientific (2 mentions)
Celltracker red cmtpx, by Thermo Fisher Scientific (2 mentions)
Tsa plus kit, by PerkinElmer (2 mentions)
Toto 3, by Thermo Fisher Scientific (2 mentions)
F4 80 clone a3 1, by Bio-Rad (2 mentions)
Mouse anti αsma clone 1a4, by Agilent Technologies (2 mentions)
Mouse anti cd68, by Agilent Technologies (2 mentions)
Rabbit anti periostin, by Abcam (2 mentions)
Prolong gold mounting media with dapi, by Thermo Fisher Scientific (1 mentions)
Rabbit anti ki67, by Abcam (1 mentions)
3 protocols using mouse anti ck19
1
Immunofluorescence Staining of Epithelial Markers
2
Multicolor Immunofluorescence Labeling
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Human tissues were labeled with the following antibodies: mouse anti-αSMA (clone 1A4, Dako, Carpineria, CA), rabbit anti-periostin (Abcam, Cambridge, ENG), mouse anti-CK19 (Dako clone RCK-108), mouse anti-CD68 (Dako clone KP1), and mouse anti-CD163 (Novocastra/Leica, New Castle Upon Tyne, ENG). Mouse tissues were labeled with the same αSMA and periostin antibodies and with F4/80 (clone A3-1, AbD Serotec, Raleigh, NC). Fibrillar collagen I was detected by Gomori trichrome (Newcomer Supply, Middleton, WI). Double immunofluorescence was performed using TSA-Plus kits (Perkin Elmer LAS, Boston, MA) and counterstained with Toto3 (Molecular Probes, Eugene, OR). For double immunofluorescence with same species antibodies, slides were boiled after the first antibody’s TSA reaction, then labeling was repeated with the second primary antibody. Controls always included slides which lacked either the first or second primary antibody to validate that boiling removed all of the first antibody.
IPS-1 cells were labeled with CellTracker Green CMFDA (Molecular Probes, Eugene OR) and RAW264.7 and BMDM-WT cells were labeled with CellTracker Red CMTPX (Molecular Probes) as per manufacturer’s instructions.
IPS-1 cells were labeled with CellTracker Green CMFDA (Molecular Probes, Eugene OR) and RAW264.7 and BMDM-WT cells were labeled with CellTracker Red CMTPX (Molecular Probes) as per manufacturer’s instructions.
3
Multicolor Immunofluorescence Labeling
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