Biological specimens were obtained from participants of both study arms (TLS/RDS). In cities where TLS was used, specimens were tested for HIV antibodies using Genscreen HIV 1/2 version 2, Bio-Rad (Marne la Roquette, France). A total IgG antibodies ELISA test Human IgG ELISA Kit 1x96, Quantitative/Immunology Consultants Laboratory was also used for oral fluid (OF) sample testing suitability and quality control. All HIV-reactive samples were re-tested with Vironostika HIV Ag/Ab, Biomerieux (Marcy-l'Étoile, France) . Samples reactive to the first ELISA HIV test, but negative to the second, were classified as negative.
In cities where RDS was used, blood specimens were collected and processed for serum extraction according to the respective national guidelines for safety and quality assurance. Serum samples were tested with a HIV fourth generation ELISA/CLIA screening test. A Western Blot test was used to confirm the positive cases.
In cities where RDS was used, blood specimens were collected and processed for serum extraction according to the respective national guidelines for safety and quality assurance. Serum samples were tested with a HIV fourth generation ELISA/CLIA screening test. A Western Blot test was used to confirm the positive cases.