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Vpp956

Manufactured by Vector Laboratories
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VPP956 is a laboratory equipment product from Vector Laboratories. It is a core component used in various scientific applications.

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Lab products found in correlation

Lysozyme, by Agilent Technologies (2 mentions) Phospho rps6 ser235 236, by Cell Signaling Technology (2 mentions) Phospho 4e bp1 thr37 46, by Cell Signaling Technology (2 mentions) Bromodeoxyuridine (brdu), by BD (2 mentions) β catenin, by BD (2 mentions) Sc1661, by Vector Laboratories (2 mentions) Phospho eef2thr56, by Novus Biologicals (2 mentions) Nb100 92518, by Santa Cruz Biotechnology (2 mentions) C myc, by Santa Cruz Biotechnology (2 mentions) Ab8580, by Abcam (1 mentions) Ab70492, by Abcam (1 mentions) Ab46540, by Abcam (1 mentions) Ab6002, by Abcam (1 mentions)

3 protocols using vpp956

1

Comprehensive Immunohistochemical Analysis

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Standard IHC techniques were used throughout this study. Antibody concentrations used were as follows: phospho-rpS6Ser235/236 (1:800; Cell Signaling 4858), phospho-4EBP1Thr37/46 (1:500; Cell Signaling 2855), phospho-eEF2Thr56 (1:500; Novus Biologicals NB100-92518), c-MYC (1:200; Santa Cruz sc-764), β-catenin (1:50; BD Biosciences 610154), BrdU (1:200; BD Biosciences 347580), lysozyme (1:150; Dako A099), GFP (1:1,000; Abcam ab6556), p21 (1/4; CNIO Madrid), p16 (1:400; Santa Cruz sc1661), p53 (1/150; Vector Laboratories VPP956). For each antibody, staining was performed on at least three mice of each genotype. Representative images are shown for each staining.
Faller W.J., Jackson T.J., Knight J.R., Ridgway R.A., Jamieson T., Karim S.A., Jones C., Radulescu S., Huels D.J., Myant K.B., Dudek K.M., Casey H.A., Scopelliti A., Cordero J.B., Vidal M., Pende M., Ryazanov A.G., Sonenberg N., Meyuhas O., Hall M.N., Bushell M., Willis A.E, & Sansom O.J. (2014). mTORC1 mediated translational elongation limits intestinal tumour initiation and growth. Nature, 517(7535), 497-500.
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2

ChIP-seq and ChIP-qPCR Protocol

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ChIP was performed as described in Extended Experimental Procedures with the following antibodies: H3K4me3 (ab8580, Abcam), H3K27me3 (ab6002, Abcam), p53 (clone CM5, VP-P956, Vector Laboratories), hnRNP-K (ab70492, Abcam), and control IgG (ChIP grade, ab46540, Abcam). DNA was submitted for high-throughput sequencing (ChIP-seq) or used for qPCR analysis (ChIP-qPCR) using primers listed in Table S4. The data represent the percentage of input that was immunoprecipitated.
Dimitrova N., Zamudio J.R., Jong R.M., Soukup D., Resnick R., Sarma K., Ward A.J., Raj A., Lee J., Sharp P.A, & Jacks T. (2014). LincRNA-p21 activates p21 in cis to promote Polycomb target gene expression and to enforce the G1/S checkpoint. Molecular cell, 54(5), 777-790.
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3

Comprehensive Immunohistochemical Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Standard IHC techniques were used throughout this study. Antibody concentrations used were as follows: phospho-rpS6Ser235/236 (1:800; Cell Signaling 4858), phospho-4EBP1Thr37/46 (1:500; Cell Signaling 2855), phospho-eEF2Thr56 (1:500; Novus Biologicals NB100-92518), c-MYC (1:200; Santa Cruz sc-764), β-catenin (1:50; BD Biosciences 610154), BrdU (1:200; BD Biosciences 347580), lysozyme (1:150; Dako A099), GFP (1:1,000; Abcam ab6556), p21 (1/4; CNIO Madrid), p16 (1:400; Santa Cruz sc1661), p53 (1/150; Vector Laboratories VPP956). For each antibody, staining was performed on at least three mice of each genotype. Representative images are shown for each staining.
Faller W.J., Jackson T.J., Knight J.R., Ridgway R.A., Jamieson T., Karim S.A., Jones C., Radulescu S., Huels D.J., Myant K.B., Dudek K.M., Casey H.A., Scopelliti A., Cordero J.B., Vidal M., Pende M., Ryazanov A.G., Sonenberg N., Meyuhas O., Hall M.N., Bushell M., Willis A.E, & Sansom O.J. (2014). mTORC1 mediated translational elongation limits intestinal tumour initiation and growth. Nature, 517(7535), 497-500.
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