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Sc 13118 fitc

Manufactured by Miltenyi Biotec
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The Sc-13118 FITC is a fluorescent antibody reagent manufactured by Miltenyi Biotec. It is designed for the detection and analysis of cellular antigens through flow cytometry applications.

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Lab products found in correlation

Stabilizing fixative, by BD (2 mentions) Epcam, by Miltenyi Biotec (2 mentions) Fluorescence microscopy, by Olympus (2 mentions)

2 protocols using sc 13118 fitc

1

Quantitative nEV Surface Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Approximately 1011 nEVs from SK-N-BE(2), MDA-MB-231 and SW620 cells, respectively, were re-suspended in 100 μl of SFM and labelled with 5 nmol of LP following the above protocol. nEVs were directly anchored onto NA coated glass substrate after incubation at RT for 30 min. All samples were fixed with 1× stabilizing fixative (BD, Biosciences) for 10 min followed by PBS rinsing thrice. Surface was blocked with 1% BSA in PBS for 30 min at RT and incubated overnight at 4 °C with the following fluorescence conjugated antibodies against CD9 (Santa Cruz Biotechnology, sc-13118 FITC) and CD326 (EpCAM, Miltenyl Biotec, 130-098-115). Afterwards, samples were thoroughly washed with PBS and observed under fluorescence microscopy (Olympus). Fluorescence intensity was quantified with ImageJ.
Wan Y., Cheng G., Liu X., Hao S.J., Nisic M., Zhu C.D., Xia Y.Q., Li W.Q., Wang Z.G., Zhang W.L., Rice S.J., Sebastian A., Albert I., Belani C.P, & Zheng S.Y. (2017). Rapid magnetic isolation of extracellular vesicles via lipid-based nanoprobes. Nature biomedical engineering, 1, 0058.
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2

Quantitative nEV Surface Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Approximately 1011 nEVs from SK-N-BE(2), MDA-MB-231 and SW620 cells, respectively, were re-suspended in 100 μl of SFM and labelled with 5 nmol of LP following the above protocol. nEVs were directly anchored onto NA coated glass substrate after incubation at RT for 30 min. All samples were fixed with 1× stabilizing fixative (BD, Biosciences) for 10 min followed by PBS rinsing thrice. Surface was blocked with 1% BSA in PBS for 30 min at RT and incubated overnight at 4 °C with the following fluorescence conjugated antibodies against CD9 (Santa Cruz Biotechnology, sc-13118 FITC) and CD326 (EpCAM, Miltenyl Biotec, 130-098-115). Afterwards, samples were thoroughly washed with PBS and observed under fluorescence microscopy (Olympus). Fluorescence intensity was quantified with ImageJ.
Wan Y., Cheng G., Liu X., Hao S.J., Nisic M., Zhu C.D., Xia Y.Q., Li W.Q., Wang Z.G., Zhang W.L., Rice S.J., Sebastian A., Albert I., Belani C.P, & Zheng S.Y. (2017). Rapid magnetic isolation of extracellular vesicles via lipid-based nanoprobes. Nature biomedical engineering, 1, 0058.
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+ Expand

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