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3 protocols using β d glucose

1

Characterization of Alginate Polymers

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Sodium alginate from Millipore Sigma (Cat# A1112) (LV), BASF (Hydagen 558P) (HV1), TIC (Algin 400) (HV2), and DuPont Danisco (GRINDSTED Alginate FD 155) (HV3) were used in this study (Table 1). Calcium hydrogen phosphate, succinic acid, sodium citrate, glacial acetic acid, sodium carbonate, β-D-glucose, sodium bicarbonate, L-serine, sodium hydroxide, ammonium hydroxide, hydrochloric acid, calcium carbonate, and methanol were purchased from Thermo Fisher. Schiff’s fuchsin sulfite reagent, sodium metabisulfate, periodic acid, anthrone, D-(+)-galacturonic acid, concentrated sulfuric acid, disodium 2,2-bicinchoninate (BCA), copper (II) sulfate, chloroform, 1-phenyl-3-methyl-5-pyrazolone (PMP), and D-(+)-mannuronic acid were purchased from Millipore Sigma. The polymannuronic acid (YP31737) and polyguluronic acid (YP03135) was obtained from Carbosynth Ltd. (Berkshire UK) at a minimum of 85% purity and 10% water content with an average molecular weight of 6–8 kDa.
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2

Hydrogel Biomaterial Composition Analysis

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High-viscosity (HV) sodium
alginate (Hydagen 558P) and sodium hyaluronate/hyaluronan (36 kDa)
were provided by BASF SE. Calcium phosphate, succinic acid, sodium
citrate, glacial acetic acid, sodium carbonate, β-d-glucose, sodium hydroxide, hydrochloric acid, calcium carbonate,
sodium citrate, phytic acid (inositol hexakisphosphate), sodium tetraborate,
sulfuric acid, and ammonium hydroxide were purchased from Thermo Fisher.
Schiff’s fuchsin sulfite reagent, sodium metabisulfate, periodic
acid, calcium chloride, citric acid, low-viscosity (LV) sodium alginate
from brown algae (catalog #A1112), and carbazole were purchased from
Millipore Sigma. Ethanol (200 proof) was purchased from Koptek. Carbon
tape and microscopy stands were purchased from Ted Pella. Ultrapure
deionized water was sourced from a MilliQ 85/15 system (Millipore
Sigma).
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3

Characterization of Sodium Alginate Variants

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Sodium alginate from Millipore Sigma (Cat# A1112) (LV), BASF (Hydagen 588P) (HV1), TIC (Algin 400) (HV2), and DuPont Danisco (GRINDSTED Alginate FD 155) (HV3) were used in this study (Table 1). Calcium phosphate, succinic acid, sodium citrate, glacial acetic acid, sodium carbonate, β-D-glucose, sodium bicarbonate, L-serine, sodium hydroxide, ammonium hydroxide, hydrochloric acid, calcium carbonate, and methanol were purchased from Thermo Fisher. Schiff's fuchsin sulfite reagent, sodium metabisulfate, periodic acid, anthrone, D-(+)-galacturonic acid, concentrated sulfuric acid, disodium 2,2-bicinchoninate (BCA), copper (II) sulfate, chloroform, 1-phenyl-3-methyl-5-pyrazolone (PMP), and D-(+)-mannuronic acid were purchased from Millipore Sigma. The polymannuronic acid (YP31737) and polyguluronic acid (YP03135) was obtained from Carbosynth Ltd. (Berkshire UK) at a minimum of 85% purity and 10% water content with an average molecular weight of 6 -8 kDa. Gelling agent for food
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