Cell titer blue h cell viability assay kit

The cell viability assay was performed using a Cell Titer-Blue H Cell Viability assay kit (Promega Corporation) according to the manufacturer's instructions. Cells were seeded at a density of 5×10³ cells/well in 96-well plates and incubated over night at 37°C. After 24 and 48 h of syntenin-targeted siRNA transfection, cell viability was determined by adding cell titer blue (20 µl/well) as an indicator and further incubated at 37°C for 4 h, fluorescence was subsequently measured at a wavelength of 560/590 nm using a Gen™ spectrophotometer (• BioTek Instruments, Inc.).

Cell proliferation was evaluated using the MTT method (Sigma-Aldrich, St. Louis, MO, USA) and Cell Titer-Blue H^® Cell Viability Assay Kit (Promega). After treatment, the cells were incubated for 0, 24, 48, and 72 h before adding the MTT reagent to each well at a final concentration of 0.5 mg/ml and incubated at 37°C for 4 h. After medium removal, 500 µl of dimethyl sulfoxide was added to each well. Viable cells were measured by absorbance at a 550 nm wavelength using a microplate reader (Bio-Rad, Hercules, CA, USA). Cell Titer-Blue H^® Cell Viability Assay was carried out according to the manufacturer’s instructions.