The WSIgE and TIgE were measured using optimized ELISA-based methods described by us previously [32 (link),42 (link),75 (link)]. In the TIgE ELISA, paired antibodies (i.e., a capture anti-mouse IgE antibody and a biotin-labeled anti-mouse IgE detection antibody) and an IgE isotype standard were used (BD Biosciences). In the SIgE ELISA, plates were coated with SSWP followed by blocking, sample additions, and then detection using a biotin-labeled anti-mouse IgE detection antibody (BD Biosciences), as described before [32 (link),42 (link)].
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