Fpquest software 4

The gel was visualized under UV light and documented using Quantity One (Bio - Rad Laboratories, France). Digital images were analyzed with FPQuest Software 4.5 (Bio - Rad Laboratories, France). The dendrogram was generated using the Dice correlation coefficient and the unweighted pair group method with arithmetic mean with 1% tolerance and 1% of band position. PFGE band patterns with ≥72% were considered and clonally related (cut off point - Sab = 72%).

All ESBL-KP isolates were analyzed using macrorestriction with XbaI (Thermo Scientific, Waltham, MA, USA), and subsequent pulsed-field gel electrophoresis (PFGE) was performed according to the protocol described by the manufacturer. The gel was visualized under UV light and documented using Quantity One (Bio-Rad, Marnes-la-Coquette, France). Digital images were analyzed with FPQuest Software 4.5 (Bio-Rad, Marnes-la-Coquette, France). The dendrogram was generated using the Dice correlation coefficient and the unweighted pair group method with arithmetic mean and 2% band tolerance.
The classification of individual restriction patterns for particular genetic profiles was carried out using the Unweighted Pair Group Method with Arithmetic Mean (UPGMA) method (SAB value = 82.5%) and the Dice coefficient (2.0%).