We obtained genomic DNA from post-mortem, homogenate tissue samples from both the discovery and replication cohorts using QIAmp DNA Mini Kits (Qiagen, Montreal, Canada) as per manufacturer specifications. Notably, DNA for the discovery cohort was extracted from the initial dissections that were used for RRBS library construction. Genomic DNA from both cohorts (2ug/sample), as well as from all sorted nuclear fractions (>100ng/sample) was converted using EpiTect Fast 96 Bisulfite Conversion Kits (Qiagen, Montreal, Canada), diluted to 150ul using MilliQ water, and stored at −20 °C.
Epitect fast 96 bisulfite conversion kits
Manufactured by Qiagen
Sourced in Canada
The EpiTect Fast 96 Bisulfite Conversion Kits are a set of laboratory equipment designed for the bisulfite conversion of DNA samples. The kits provide a fast and efficient method for converting unmethylated cytosine residues into uracil, while leaving methylated cytosines unchanged, which is a crucial step in the analysis of DNA methylation patterns.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using epitect fast 96 bisulfite conversion kits
1
DNA Extraction and Bisulfite Conversion
2
DNA Extraction and Bisulfite Conversion
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
We obtained genomic DNA from post-mortem, homogenate tissue samples from both the discovery and replication cohorts using QIAmp DNA Mini Kits (Qiagen, Montreal, Canada) as per manufacturer specifications. Notably, DNA for the discovery cohort was extracted from the initial dissections that were used for RRBS library construction. Genomic DNA from both cohorts (2ug/sample), as well as from all sorted nuclear fractions (>100ng/sample) was converted using EpiTect Fast 96 Bisulfite Conversion Kits (Qiagen, Montreal, Canada), diluted to 150ul using MilliQ water, and stored at −20 °C.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!