310 ms lc ms ms triple quadrupole mass spectrometer

All reagents, solvents, and chemical materials were purchased from Merck and Chimopar and used as received. Compounds 1 and 5 were commercially available; compounds 2–4, and 8 are known in the literature and we therefore synthesized them following similar methods [14 (link),15 (link),16 (link),17 (link)]; compounds 7 and 9 are new derivatives obtained, and their physico-chemical characteristics are presented in detail. The purity of all compounds was checked with TLC (single spot).
UV-Vis measurements were performed in methanol, using a UVD-3500 UV-Vis double-bean spectrophotometer.
IR spectra were measured using a Bruker Tensor 27 FT-IR spectrometer.
NMR spectra were measured in chloroform-d₁ or DMSO-d₆ using a Bruker Advance spectrometer operating at 500 MHz for ¹H and 125 MHz for ¹³C. We report the chemical shifts δ as ppm values and the residual solvent peaks were used as an internal reference. For the MS spectra, we used a Varian 310—MS LC/MS/MS triple quadrupole mass spectrometer fitted with an electrospray ionization interface (ESI).

IR spectra were measured using a Bruker Tensor 27 FT-IR spectrometer (ATR). UV-Vis measurements were performed in DCM using a Cary 4000 UV-Vis Agilent Technologies spectrophotometer. Fluorescence measurements were performed in DCM using a lifetime and steady-state spectrometer-FLSP 920, Edinburgh Instruments. Regarding the NMR spectra, these were measured in chloroform-d1 or DMSO-d6 using a Bruker Advance spectrometer operating at 500 MHz for ¹H and 125 MHz for ¹³C. As usual, the chemical shifts are reported as δ ppm values, and the residual solvent peaks were used as the internal reference. A Varian 310—MS LC/MS/MS triple quadrupole mass spectrometer fitted with an electrospray–ionisation interface (ESI) was used for the MS spectra. The ESR spectrum was recorded on a Jes-FA 100 Jeol Instrument. For the X-ray diffraction measurements for compound 3 employed a Rigaku XtaLAB Synergy-S diffractometer operating with an Mo-K (λ = 0.71073 Å) micro-focus sealed X-ray tube. The structure was solved using direct methods and refined by full-matrix least-squares techniques based on F². The non-H atoms were refined with anisotropic displacement parameters. The SHELX-2018 crystallographic software package was used for calculations. The crystallographic data summary and the structure refinement for compound 3 are given in Table 3. CCDC reference number: 2280342.