Human fc blocking solution

Manufactured by BioLegend

Sourced in United States

BioLegend's Human FC Blocking Solution is a laboratory reagent designed to block Fc receptors on human cells, preventing non-specific binding of Fc-containing antibodies or proteins. The solution contains a proprietary formulation to effectively block Fc receptors without interfering with the binding of target-specific antibodies.

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Lab products found in correlation

Alexa fluor 488 donkey anti goat secondary antibody, by Thermo Fisher Scientific (2 mentions) Fortessa flow cytometer, by BD (1 mentions)

Close spelling variants of this product

Human TruStain FcX Fc receptor blocking solution (60 citations) Human Fc receptor blocking solution (19 citations) Fc blocking solution (8 citations) Human TruStain FcX Fc blocking solution (5 citations) Human TruStain Fc Receptor Blocking Solution (2 citations)

3 protocols using human fc blocking solution

Quantifying Lymphocyte Subsets by FACS

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Changes in lymphocyte subsets in blood, the spleen and LNs were quantified by Fluorescence-Activated Cell Sorting (FACS) analysis. Multicolor FACS analysis was performed to measure the T cells, B-cell depletion/repletion kinetics. Prior to staining, cells were washed in FACS buffer (PBS containing 2% bovine serum albumin [BSA], 5 mM EDTA), then blocked with a human Fc blocking solution (Biolegend, USA), and stained for 30 min at 4°C with the indicated combination of fluorochrome or biotin conjugated antibodies. When required, a second incubation for 30 min at 4°C was performed with the secondary antibody. Washed and resuspended cells were acquired on a Fortessa flow cytometer (Becton Dickinson, USA). Data were analyzed using FlowJo software (Flow Jo LLC, USA). The flow cytometry antibodies were obtained from commercial sources listed in Supplementary Table 2. The flow cytometry gating strategy is outlined in Table 1.
Parametric unpaired student's t-test was performed for analyzing the flow cytometry data using GraphPad Prism (version 4.00; GraphPad Software, San Diego, California, USA). A value of p < 0.05 was considered statistically significant.

Theil D., Smith P., Huck C., Gilbart Y., Kakarieka A., Leppert D., Rauld C., Schmid C., Baumgartner R., Stuber N., Cordoba F., Dubost V., Darribat K., Jivkov M., Frieauff W., Kneuer R., Stoeckli M., Reinker S., Mansfield K., Carballido J.M., Couttet P, & Weckbecker G. (2019). Imaging Mass Cytometry and Single-Cell Genomics Reveal Differential Depletion and Repletion of B-Cell Populations Following Ofatumumab Treatment in Cynomolgus Monkeys. Frontiers in Immunology, 10, 1340.

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ACE2 Cell Surface Analysis by Flow Cytometry

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For cell surface analysis of ACE2, we harvested cells and washed them in fluorescence-activated cell sorting (FACS) buffer (2% FBS in 1× PBS). Cells were resuspended in a 1:50 dilution of human F_C blocking solution (number 422302; BioLegend) and incubated on ice for 10 min. Human ACE2 antibody or goat IgG isotype control was then added to the cells to obtain the final concentration of 5 μg/ml followed by 1 h of incubation on ice. The cells were washed with FACS buffer and incubated for 30 min on ice in the dark with a 1:400 dilution of Alexa Fluor 488 donkey anti-goat secondary antibody (number A11055; Invitrogen). The cells were washed and resuspended in FACS buffer. Data were collected using a BD LSR II flow cytometer and analyzed with FlowJo software (version 10).

Chen D.Y., Khan N., Close B.J., Goel R.K., Blum B., Tavares A.H., Kenney D., Conway H.L., Ewoldt J.K., Chitalia V.C., Crossland N.A., Chen C.S., Kotton D.N., Baker S.C., Fuchs S.Y., Connor J.H., Douam F., Emili A, & Saeed M. (2021). SARS-CoV-2 Disrupts Proximal Elements in the JAK-STAT Pathway. Journal of Virology, 95(19), e00862-21.

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ACE2 Expression Analysis by Flow Cytometry

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For cell surface analysis of ACE2, we harvested cells and washed them in FACS Buffer (2% FBS in 1X PBS). Cells were resuspended in 1:50 dilution of human F_C blocking solution (BioLegend; #422302) and incubated on ice for 10 min. Human ACE2 antibody or goat IgG isotype control was then added to the cells to obtain the final concentration of 5 μg/mL followed by 1h incubation on ice. The cells were washed with FACS buffer and incubated for 30 minutes on ice in the dark with 1:400 dilution of Alexa Fluor 488 donkey anti-goat secondary antibody (Invitrogen; #A11055). The cells were washed and resuspended in FACS buffer. Data were collected using a BD LSR II flow cytometer and analyzed with FlowJo software (version 10).

Chen D.Y., Turcinovic J., Feng S., Kenney D.J., Chin C.V., Choudhary M.C., Conway H.L., Semaan M., Close B.J., Tavares A.H., Seitz S., Khan N., Kapell S., Crossland N.A., Li J.Z., Douam F., Baker S.C., Connor J.H, & Saeed M. (2023). Cell culture systems for isolation of SARS-CoV-2 clinical isolates and generation of recombinant virus. iScience, 26(5), 106634.

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