Alanine aminotransferase activity kit

Manufactured by Abcam

The Alanine Aminotransferase Activity kit is a laboratory tool used to measure the activity of the enzyme alanine aminotransferase (ALT) in biological samples. ALT is an important enzyme involved in amino acid metabolism and its activity is commonly used as a biomarker for liver function.

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Lab products found in correlation

Nefa kit, by Fujifilm (2 mentions) Mouse ultrasensitive insulin elisa kit, by Crystal Chem (2 mentions) Infinity triglycerides kit, by Thermo Fisher Scientific (2 mentions) Aspartate aminotransferase activity kit, by Abcam (2 mentions) Mouse leptin elisa, by Crystal Chem (2 mentions)

2 protocols using alanine aminotransferase activity kit

Plasma Metabolic Biomarker Analysis

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Whole blood was taken from the facial vein and blood glucose was measured with a glucose meter (Easy Touch) from the tail vein. Plasma was collected after centrifugation at 1,200 x rpm, 4°C for 10 min. Plasma triglyceride (TG) and free fatty acid (FFA) levels were measured with Infinity^™ Triglycerides kit (Thermo Fisher) and NEFA kit (WAKO). Plasma insulin levels were measured with the Mouse Ultrasensitive Insulin ELISA kit (Crystal Chem, #90080), and leptin levels were measured with Mouse Leptin ELISA (Crystal Chem, #90030) kit. Plasma AST and ALT activity was measured with the Aspartate Aminotransferase Activity kit (Biovision, #K753) and Alanine Aminotransferase Activity kit (Biovision, #K752).

xia W., Veeragandham P., Cao Y., Xu Y., Rhyne T., Qian J., Hung C.W., Zhao P., Jones Y., Gao H., Liddle C., Yu R., Downes M., Evans R., Ryden M., Wabitsch M., Reilly S., Huang J, & Saltiel A. (2023). Obesity-dependent increase in RalA activity disrupts mitochondrial dynamics in white adipocytes. Research Square.

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Comprehensive Metabolic Profiling of Mice

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Whole blood was taken from the facial vein and blood glucose was measured with a glucose meter (Easy Touch) from the tail vein. Plasma was collected after centrifugation at 1,200g at 4 °C for 10 min. Plasma TG and FFA levels were measured with an Infinity Triglycerides kit (Thermo Fisher) and NEFA kit (WAKO). Plasma insulin levels were measured with the Mouse Ultrasensitive Insulin ELISA kit (Crystal Chem, 90080) and leptin levels were measured with a Mouse Leptin ELISA (Crystal Chem, 90030) kit. Plasma AST and ALT activity was measured with the Aspartate Aminotransferase Activity kit (Biovision, K753) and Alanine Aminotransferase Activity kit (Biovision, K752).

Xia W., Veeragandham P., Cao Y., Xu Y., Rhyne T.E., Qian J., Hung C.W., Zhao P., Jones Y., Gao H., Liddle C., Yu R.T., Downes M., Evans R.M., Rydén M., Wabitsch M., Wang Z., Hakozaki H., Schöneberg J., Reilly S.M., Huang J, & Saltiel A.R. (2024). Obesity causes mitochondrial fragmentation and dysfunction in white adipocytes due to RalA activation. Nature Metabolism, 6(2), 273-289.

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