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Aggrewell 400 24

Manufactured by STEMCELL
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The Aggrewell 400 24 is a 24-well plate designed for the formation of 3D cell aggregates, or spheroids, from single-cell suspensions. The plate features microwells that facilitate the rapid formation of uniform, high-quality spheroids.

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Lab products found in correlation

Anti adherence rinsing solution, by STEMCELL (2 mentions) Aggrewell eb formation medium, by STEMCELL (2 mentions) Tesr e6 media, by STEMCELL (2 mentions) Cls3471 24ea, by Corning (2 mentions) Gentle cell dissociation reagent (gcdr), by STEMCELL (2 mentions) Aggrewell 400, by STEMCELL (2 mentions)

2 protocols using aggrewell 400 24

1

Embryoid Body Formation using Aggrewell

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Embryoid bodies (EB) were formed using the Aggrewell-400 protocol according to the manufacturer’s protocol (STEMCELL Technologies). Briefly, the iPSCs were dissociated with Gentle Cell Dissociation Reagent (100–0485, STEMCELL Technologies) and seeded into the Aggrewell 400 24-well plate pre-coated with the anti-adherence rinsing solution (07010, STEMCELL Technologies) at a density of 1.2e6 cells/well in Aggrewell EB Formation Medium (05893, STEMCELL Technologies). After 24hr, half of the media was replaced with fresh Aggrewell EB Formation Medium. 48 hr after seeding, we harvested EBs and moved them to an ultra-low attachment 6-well plate (CLS3471-24EA, Corning) in TeSR™-E6 media (05946, STEMCELL Technologies). We maintained EBs in culture for 6 days, replacing media every other day.
Metzl-Raz E., Bharucha N., Ataam J.A., Gavidia A.A., Greenleaf W.J, & Karakikes I. (2022). Generation of a dual edited human induced pluripotent stem cell Myl7-GFP reporter line with inducible CRISPRi/dCas9. Stem cell research, 61, 102754.
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2

Embryoid Body Formation using Aggrewell

Check if the same lab product or an alternative is used in the 5 most similar protocols
Embryoid bodies (EB) were formed using the Aggrewell-400 protocol according to the manufacturer’s protocol (STEMCELL Technologies). Briefly, the iPSCs were dissociated with Gentle Cell Dissociation Reagent (100–0485, STEMCELL Technologies) and seeded into the Aggrewell 400 24-well plate pre-coated with the anti-adherence rinsing solution (07010, STEMCELL Technologies) at a density of 1.2e6 cells/well in Aggrewell EB Formation Medium (05893, STEMCELL Technologies). After 24hr, half of the media was replaced with fresh Aggrewell EB Formation Medium. 48 hr after seeding, we harvested EBs and moved them to an ultra-low attachment 6-well plate (CLS3471-24EA, Corning) in TeSR™-E6 media (05946, STEMCELL Technologies). We maintained EBs in culture for 6 days, replacing media every other day.
Metzl-Raz E., Bharucha N., Ataam J.A., Gavidia A.A., Greenleaf W.J, & Karakikes I. (2022). Generation of a dual edited human induced pluripotent stem cell Myl7-GFP reporter line with inducible CRISPRi/dCas9. Stem cell research, 61, 102754.
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