Slan m dc8 monocyte isolation kit

Manufactured by Miltenyi Biotec

Sourced in France

The Slan-(M-DC8)+ Monocyte Isolation Kit is a lab equipment product designed for the isolation of Slan-(M-DC8)+ monocytes from human peripheral blood mononuclear cells (PBMCs). The kit utilizes magnetic beads coated with antibodies specific to the Slan-(M-DC8) surface marker to selectively isolate the target cell population.

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Lab products found in correlation

Ficoll gradient, by Eurobio Scientific (1 mentions)

Close spelling variants of this product

Monocyte Isolation Kit (79 citations) Isolation kits (10 citations)

2 protocols using slan m dc8 monocyte isolation kit

Isolation of NK cells and non-classical monocytes

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Peripheral blood mononuclear cells (PBMCs) were isolated from the blood of healthy volunteers from the Etablissement Français du Sang (Besançon, France) by Ficoll gradient centrifugation (Eurobio, Courtaboeuf, France). NK cells were purified from PBMCs by negative selection with magnetic enrichment kit (Stemcell, Grenoble, France) and FcγIII+ CD14- non-classical monocytes were isolated from PBMCs using Slan-(M-DC8)+ Monocyte Isolation Kit, (Miltenyi Biotec, Paris, France)⁸². All sorted cell populations exhibited high purity (>90%), as revealed by flow cytometry.

Lamarthée B., Callemeyn J., Van Herck Y., Antoranz A., Anglicheau D., Boada P., Becker J.U., Debyser T., De Smet F., De Vusser K., Eloudzeri M., Franken A., Gwinner W., Koshy P., Kuypers D., Lambrechts D., Marquet P., Mathias V., Rabant M., Sarwal M.M., Senev A., Sigdel T.K., Sprangers B., Thaunat O., Tinel C., Van Brussel T., Van Craenenbroeck A., Van Loon E., Vaulet T., Bosisio F, & Naesens M. (2023). Transcriptional and spatial profiling of the kidney allograft unravels a central role for FcyRIII+ innate immune cells in rejection. Nature Communications, 14, 4359.

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Isolation of Slan-Monocytes from PBMCs

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Peripheral blood mononuclear cells (PBMCs) were isolated with a gradient centrifugation procedure on a lymphocyte separation medium (Lymphosep; Dutscher, Issy -les-Moulineaux, France). SlanMo were isolated from PBMCs using the Slan-(M-DC8)+ Monocyte Isolation Kit (Miltenyi), according to the manufacturer's instructions. Slanmo purity was always higher than 75%.

Ricard L., Eshagh D., Siblany L., de Vassoigne F., Malard F., Laurent C., Beurier P., Jachiet V., Rivière S., Fain O., Mohty M., Gaugler B, & Mekinian A. (2022). 6-Sulfo LacNAc monocytes are quantitatively and functionally disturbed in systemic sclerosis patients. Clinical and experimental immunology, 209(2).

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