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Mouse human fcr blocker

Manufactured by Miltenyi Biotec
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The 1X mouse/human FcR blocker is a laboratory reagent designed to block Fc receptors on cells. It is suitable for use with both mouse and human cells. The blocker helps to prevent non-specific binding of antibodies to Fc receptors, which is important for various immunological applications.

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Lab products found in correlation

Cleaved caspase 3 asp175 5a1e, by Cell Signaling Technology (2 mentions) Anti ki67 sp6, by Thermo Fisher Scientific (2 mentions) S1699 retrieval buffer, by Agilent Technologies (2 mentions) Dab staining kit, by Thermo Fisher Scientific (2 mentions)

2 protocols using mouse human fcr blocker

1

Immunohistochemistry of Apoptosis Markers

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Deparaffinized and rehydrated formalin fixed samples tumor samples were subjected to antigen retrieval using Dako S1699 Retrieval Buffer at pH 6.1 at 90oC for 30 mins. After a 5 min peroxidase blockade, slides were blocked for 10 min at RT with PBS containing 0.5% BSA, 5% goat serum, and 1X mouse/human FcR blocker (Miltenyi Biotec 130–092-575). Slides were incubated with primary anti-Bnip3 (EPR4034, Abcam) at 1:100 at RT for 30 min, rabbit mAb to cleaved Caspase-3 (Asp175) (5A1E) (Cell Signaling Technology) or anti-Ki67 (SP6) (Thermo-Scientific) at 1:100 overnight at 4oC. IHC was visualized by DAB staining kit (TL-015-HD by Invitrogen), counterstained with hematoxylin, and mounted in non-aqueous solution (Richard-Allen Scientific 4112).
Nollet E.A., Cardo-Vila M., Ganguly S.S., Tran J.D., Schulz V.V., Cress A., Corey E, & Miranti C.K. (2020). Androgen Receptor-Induced Integrin α6β1 and Bnip3 Promotes Survival and Resistance to PI3K Inhibitors in Castration-Resistant Prostate Cancer. Oncogene, 39(31), 5390-5404.
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2

Immunohistochemistry of Apoptosis Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols
Deparaffinized and rehydrated formalin fixed samples tumor samples were subjected to antigen retrieval using Dako S1699 Retrieval Buffer at pH 6.1 at 90oC for 30 mins. After a 5 min peroxidase blockade, slides were blocked for 10 min at RT with PBS containing 0.5% BSA, 5% goat serum, and 1X mouse/human FcR blocker (Miltenyi Biotec 130–092-575). Slides were incubated with primary anti-Bnip3 (EPR4034, Abcam) at 1:100 at RT for 30 min, rabbit mAb to cleaved Caspase-3 (Asp175) (5A1E) (Cell Signaling Technology) or anti-Ki67 (SP6) (Thermo-Scientific) at 1:100 overnight at 4oC. IHC was visualized by DAB staining kit (TL-015-HD by Invitrogen), counterstained with hematoxylin, and mounted in non-aqueous solution (Richard-Allen Scientific 4112).
Nollet E.A., Cardo-Vila M., Ganguly S.S., Tran J.D., Schulz V.V., Cress A., Corey E, & Miranti C.K. (2020). Androgen Receptor-Induced Integrin α6β1 and Bnip3 Promotes Survival and Resistance to PI3K Inhibitors in Castration-Resistant Prostate Cancer. Oncogene, 39(31), 5390-5404.
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