Unity inova 500 spectrometer

Manufactured by Bruker

The UNITY INOVA 500 spectrometer is a high-performance nuclear magnetic resonance (NMR) instrument designed for analytical and research applications. It provides a core function of generating and detecting radio-frequency signals to analyze the magnetic properties of atomic nuclei within a sample. The instrument is capable of operating at a magnetic field strength of 11.74 Tesla, enabling high-resolution NMR spectroscopy.

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Lab products found in correlation

Series 2795 system, by Waters Corporation (2 mentions) Amx 500 spectrometer, by Bruker (2 mentions) Luna c18, by Phenomenex (1 mentions) Luna 5 μ c18 2 100 a column, by Phenomenex (1 mentions) 5989b gc ms spectrometer, by Hewlett-Packard (1 mentions) Sephadex lh 20, by GE Healthcare (1 mentions)

Close spelling variants of this product

Unity INOVA-500 INOVA-500 spectrometer Unity Inova Inova 500 Unity 500 Inova spectrometer 500 Spectrometers Bruker spectrometers

2 protocols using unity inova 500 spectrometer

Spectroscopic Techniques for Compound Analysis

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Nuclear magnetic resonance (NMR) spectra were measured on a Varian UNITY INOVA 500 spectrometer or on a Bruker AMX 500 spectrometer operating at 500 MHz for ¹H-NMR and 125 MHz for ¹³C-NMR with chemical shifts given in ppm (δ). Electrospray ionization-tandem mass spectrometry (ESI-MS) was measured on an Agilent 1100 liquid chromatography/mass spectrometry (LC/MS) spectrometer with a Phenomenex Luna C18 analytical column (5 μm, 4.6 × 100 mm). The high performance liquid chromatography (HPLC) apparatus (Waters Alliance series 2795 system) was composed of a vacuum degasser, a quaternary pump, a photodiode array detector, an auto-injector, and a column compartment with a thermostat. Flash and open column chromatography was carried out on Merck silica gel 60 (70–230 mesh). Reversed-phase HPLC was performed on a Gilson 321 pump system using Phenomenex Luna C18 column (10 μm, 10 × 250 mm) with a flow rate of 4 ml/min or 2 ml/min.

Yoon J.Y., Kim J.H., Baek K.S., Kim G.S., Lee S.E., Lee D.Y., Choi J.H., Kim S.Y., Park H.B., Sung G.H., Lee K.R., Cho J.Y, & Noh H.J. (2015). A direct protein kinase B-targeted anti-inflammatory activity of cordycepin from artificially cultured fruit body of Cordyceps militaris. Pharmacognosy Magazine, 11(43), 477-485.

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Spectroscopic Analysis of Compounds

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All of the NMR spectra were measured on a Varian UNITY INOVA 500 spectrometer or on a Bruker AMX 500 spectrometer operating at 500 MHz for ¹H-NMR and 125 MHz for ¹³C-NMR in DMSO-d₆ using TMS as an internal standard. EI-MS was measured on a Hewlett Packard model 5989B GC/MS spectrometer. The HPLC apparatus (Waters Alliance series 2795 system) was composed of a vacuum degasser, a quaternary pump, a photodiode array detector (PDA), an autoinjector, and a column compartment with a thermostat. A Luna 5 μ C18(2) 100 A column (4.6 × 150 mm 5 μm, Phenomenex) was used for isolation and purification of the compounds. A silica gel (70–230 mesh, Merck) and a Sephadex LH-20 (GE Healthcare) were used for open column chromatography. TLC was performed on silica gel (60 F₂₅₄, Merck).

Kim J.H., Lee Y., Sung G.H., Kim H.G., Jeong D., Park J.G., Baek K.S., Sung N.Y., Yang S., Yoon D.H., Lee S.Y., Kang H., Song C., Cho J.H., Lee K.H., Kim T.W, & Cho J.Y. (2015). Antiproliferative and Apoptosis-Inducing Activities of 4-Isopropyl-2,6-bis(1-phenylethyl)phenol Isolated from Butanol Fraction of Cordyceps bassiana. Evidence-based Complementary and Alternative Medicine : eCAM, 2015, 739874.

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