β glycerol

Manufactured by Merck Group

Sourced in Macao

β-glycerol is a chemical compound used in laboratory settings. It serves as a component in various buffer solutions and growth media. The core function of β-glycerol is to maintain the pH and osmotic balance in experimental conditions.

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Lab products found in correlation

Dexamethasone (dex), by Merck Group (2 mentions) C0745, by Merck Group (2 mentions) Histone h1, by Merck Group (2 mentions) Mgcl2, by Merck Group (2 mentions) Ethylene glycol tetraacetic acid (egta), by Merck Group (2 mentions) Ethylene diamine tetraacetic acid (edta), by Merck Group (2 mentions) Ascorbic acid, by Merck Group (1 mentions) Cellstar, by Greiner (1 mentions) Na3vo4, by Merck Group (1 mentions)

Close spelling variants of this product

Glycerol (1988 citations) β-glycerol phosphate (419 citations) β-glycerol phosphate disodium salt pentahydrate (7 citations) β-glycerol phosphate (βGP; (7 citations) β-glycerol phosphate sodium (6 citations) β-glycerol-2-phosphate (5 citations) Glycerol 2-phosphate disodium salt hydrate (β-GP) (4 citations) β-glycerol phosphoric acid (2 citations) β-glycerol phosphate disodium salt (2 citations)

5 protocols using β glycerol

Chicken and Rat BM-MSC Osteogenic Differentiation

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Chicken BM-MSCs were seeded in 4 or 24-well plates (Thermo Scientific; CELLSTAR^®, Greiner bio-one) at a density of 2.5 × 10⁴ cells/cm² and cultured in complete media (CM, Dulbecco's modified Eagle Media (DMEM, Life Technologies) containing 10% heat-inactivated fetal bovine serum, 0.3 mg/mL glutamine, 100 units/mL penicillin, and 100 μg/mL streptomycin and 1.8mM calcium chloride) until cells reached 100% confluence. Rat BM-MSCs were used as a positive control and for comparison. Osteogenic differentiation was induced by supplementing the cells with osteogenic induction media (IM), complete media containing 10mM β-glycerol (Sigma-Aldrich), 0.05mM ascorbic acid (Sigma-Aldrich) and 100nM dexamethasone (Sigma-Aldrich). Media was changed every 3 d alternating between induction and maintenance media containing 2.4mM (IM+2.4nM) or 24nM (IM+24nM) of the active vitamin D₃ metabolite 1,25(OH)₂D₃, Sigma-Aldrich)_. Control cultures for both chicken and rat BM-MSCs were designated as those cultured only in complete media (CM) containing no inducing compounds. No Treatment control cultures received induction media (IM) only and contained no exogenous 1,25(OH)₂D₃. Chicken and rat cells were cultured for a maximum of 7 and 21 d post induction, respectively.

Pande V.V., Chousalkar K.C., Bhanugopan M.S, & Quinn J.C. (2015). Super pharmacological levels of calcitriol (1,25-(OH)2D3) inhibits mineral deposition and decreases cell proliferation in a strain dependent manner in chicken mesenchymal stem cells undergoing osteogenic differentiation in vitro. Poultry Science, 94(11), 2784-2796.

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Pimecrolimus-Induced Phosphoproteomic Analysis

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MeWo cells were treated with vehicle control DMSO or pimecrolimus (10 μM) for 2 hrs before total cellular proteins were extracted by 2% SDS, Tris buffer, pH 7.6, in the presence of phosphatase inhibitors [20 mM NaF (Sigma Aldrich), 1 mM Na₃VO₄ (Sigma Aldrich), and 1mM β-glycerol (Sigma Aldrich)]. Three biological replicate samples for each condition were analyzed by Zr-IMAC phosphopeptide enrichment, followed by Orbitrap mass spectrometry performed in Stanford University Mass Spectrometry facility.

Zhou M., Kamarshi V., Arvin A.M, & Oliver S.L. (2020). Calcineurin phosphatase activity regulates Varicella-Zoster Virus induced cell-cell fusion. PLoS Pathogens, 16(11), e1009022.

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Cdk5/p25-Mediated Histone H1 Phosphorylation

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Static activity measurements of Cdk5/p25 phosphorylation of histone H1 were performed by suspending 100 ng of the enzyme (C0745; Sigma Aldrich, MO) in 50 μL of 1× kinase buffer to obtain a final concentration of 18.5 nM. The Cdk5/p25 concentration was reduced five-fold for dynamic measurements to obtain a final concentration of 3.7 nM. A stock solution of 5× kinase buffer was prepared by suspending 25 mM β-glycerol (G9422; Sigma Aldrich, MO), 50 mM MgCl₂ (5980; Millipore, MA), 5 mM EGTA (E0396; Sigma Aldrich, MO), 2.4 mM EDTA (1002264786; Sigma Aldrich, MO), 1.25 mM MOPS (M1254; Sigma Aldrich, MO) in deionized water (DIW) and diluting further to form 1× kinase buffer. Solutions of the substrate protein were prepared by adding 2 mg/mL of histone H1 (10223549001; Sigma Aldrich, MO) to the assay to obtain the final concentrations as described in the main text. The phosphorylation reaction was triggered with a cocktail of DTT and ATP. The final concentration of the ATP and DTT solution was adjusted to 250 μM and 5 mM in DIW respectively.

Le S.T., Guros N.B., Bruce R.C., Cardone A., Amin N.D., Zhang S., Klauda J.B., Pant H.C., Richter C.A, & Balijepalli A. (2019). Quantum Capacitance-Limited MoS2 Biosensors Enable Remote Label-Free Enzyme Measurements. Nanoscale, 11(33), 15622-15632.

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Cdk5/p25 Kinase Activity Assay

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The activity of Cdk5/p25 was estimated by measuring the phosphorylation of histone H1 as reported previously.^{8 (link)} All measurements were performed with 18.5 nM of Cdk5/p25 (C0745; Sigma Aldrich, MO) in 1× kinase buffer to match physiological conditions using a volume of 50 μL. The substrate protein histone H1 (10223549001; Sigma Aldrich, MO) was suspended in deionized water at a stock concentration of 2 mg/mL and further diluted as described in the in the Results section. Substrate phosphorylation was initiated with a mixture of dithiothreitol (DTT) and adenosine triphosphate (ATP) with final concentrations of 250 μM and 5 mM respectively. The measurements were buffered using 5× kinase buffer, prepared by suspending 25 mM β-glycerol (G9422; Sigma Aldrich, MO), 50 mM MgCl₂ (5980; Millipore, MA), 5 mM EGTA (E0396; Sigma Aldrich, MO), 2.4 mM EDTA (1002264786; Sigma Aldrich, MO), 1.25 mM MOPS (M1254; Sigma Aldrich, MO) in deionized water (DIW). The kinase buffer was then diluted in DIW to form the 1× kinase buffer used in the assays.

Le S.T., Morris M.A., Cardone A., Guros N.B., Klauda J.B., Sperling B.A., Richter C.A., Pant H.C, & Balijepalli A. (2020). Rapid, Quantitative Therapeutic Screening for Alzheimer’s Enzymes Enabled by Optimal Signal Transduction with Transistors. The Analyst, 145(8), 2925-2936.

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Osteogenic Differentiation Protocol

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Untreated or MI192 pre-treated cells were cultured in osteogenic medium, consisting of basal medium supplemented with 50 µM L-ascorbic acid 2-phosphate sesquimagnesium salt hydrate, 10 mM βglycerol phosphate and 100 nM dexamethasone (Sigma-Aldrich, UK). The medium was changed every 3 days.

Man K., Mekhileri N.V., Lim K.S., Jiang L.H., Woodfield T.B.F, & Yang X.B. (2021). MI192 induced epigenetic reprogramming enhances the therapeutic efficacy of human bone marrows stromal cells for bone regeneration. Bone, 153.

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