Fructose syrup (55%) was purchased from Chao Khun Agro Products Co., Ltd. (Bangkok, Thailand). Bovine serum albumin (BSA), 5,5-dithiobis-(2-nitrobenzoic acid) (DTNB), glutathione reductase, oxidized glutathione (GSSG), reduced glutathione (GSH), nitrotetrazolium blue chloride (NBT), reduced β-nicotinamide adenine dinucleotide phosphate (NADPH), standard malondialdehyde (MDA), standard superoxide dismutase (SOD) from bovine erythrocytes, 4-vinylpyridine (4-VP), and xanthine oxidase were from Sigma Aldrich (St. Louis, MO, USA). Hydrogen peroxide (H2O2) was obtained from Fisher Scientific (Leicestershire, UK). 2-Thiobarbituric acid (TBA) was supplied by Fluka Chemika Co. (Steinheim, Switzerland). Trizol® was supplied by Invitrogen® (Carlsbad, CA, USA). ReverTraAce® and Taq DNA polymerase were purchased from Toyobo Co., Ltd. (Osaka, Japan) and Vivantis Technologies Sdn. Bhd. (Selangor Darul Ehsan, Malaysia), respectively. The random primers and RNase inhibitor were products of Takara Bio Inc. (Shiga, Japan). The forward and reverse primers for CAT, CuZn-SOD, Mn-SOD, GPx, and GAPDH (Table 1 ) were synthesized by Bio Basic, Inc. (Markham, Ontario, Canada). All other laboratory chemicals were of the highest available purity from commercial suppliers.
Gapdh
Manufactured by Bio Basic
Sourced in Canada
GAPDH is a glycolytic enzyme that catalyzes the sixth step of glycolysis, the reversible oxidation of glyceraldehyde-3-phosphate to 1,3-bisphosphoglycerate. It is a key enzyme involved in energy production through the breakdown of glucose.
Automatically generated - may contain errors
Lab products found in correlation
Reduced glutathione, by Merck Group (1 mentions)
Oxidized glutathione, by Merck Group (1 mentions)
Random primer, by Takara Bio (1 mentions)
Rnase inhibitor, by Takara Bio (1 mentions)
Bovine serum albumin (bsa), by Merck Group (1 mentions)
Trizol, by Thermo Fisher Scientific (1 mentions)
Hydrogen peroxide h2o2, by Thermo Fisher Scientific (1 mentions)
Xanthine oxidase, by Merck Group (1 mentions)
Nitrotetrazolium blue chloride, by Merck Group (1 mentions)
5 5 dithiobis 2 nitrobenzoic acid, by Merck Group (1 mentions)
Revertra ace, by Toyobo (1 mentions)
Standard malondialdehyde, by Merck Group (1 mentions)
4 vinylpyridine 4 vp, by Merck Group (1 mentions)
Taq dna polymerase, by Toyobo (1 mentions)
2 protocols using gapdh
1
Antioxidant Enzyme Assay Protocol
2
Quantitative Analysis of TGF-β Expression
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Extraction of RNA from formalin-fixed samples and subsequent cDNA synthesis were performed from the tissue of normal esophagus, BE, and cardiac epithelium. PCR reactions were performed in duplicate for each sample, with first strand cDNA synthesis kit (Thermo, RevertAid™). Expression of TGF-β was determined with primers and probe supplied by Applied Biosystems. The target gene expression was normalized against mRNA expression of the housekeeping gene glyceraldehyde-3-phosphate dehydrogenase (GAPDH, Bio Basic Inc.). For each sample under investigation, the amount of target gene mRNA and GAPDH mRNA was determined from a standard curve.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!