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Iq sybr green supermix on myiq real time pcr detection system

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The IQ SYBR Green Supermix is a ready-to-use solution designed for real-time PCR detection using the MyiQ real-time PCR detection system. It contains SYBR Green I dye, which binds to double-stranded DNA and emits fluorescence upon binding, enabling the monitoring of DNA amplification in real-time.

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Lab products found in correlation

Quantitect reverse transcription kit, by Qiagen (3 mentions) Trizol, by Thermo Fisher Scientific (2 mentions)

Spelling variants of this product

MyiQ Real-Time PCR Detection System (121 citations) MyiQ Real-Time PCR System (59 citations) SYBR green real time PCR (10 citations) MyiQ real-time detection system (6 citations) MyiQ real-time system (5 citations) IQ SYBR green real-time supermix (4 citations) Real-time PCR SYBR Green detection system (4 citations) MyiQ real-time PCR (3 citations) SYBR green real-time PCR system (2 citations) IQ SYBR green detection system (2 citations)

3 protocols using iq sybr green supermix on myiq real time pcr detection system

1

Quantitative RT-PCR of GCLC Expression

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Proximal small intestine, distal small intestine and colon were isolated from mice of the desired ages into Trizol (Invitrogen, Grand Island, NY). Samples were briefly sonicated and total RNA isolated and reverse transcribed from random hexamer primers using the QuantiTect Reverse Transcription Kit (Qiagen, Carol Stream, IL). The resulting cDNA products were analyzed by real-time quantitative RT-PCR (iQ SYBR Green Supermix on MyiQ real time PCR detection system, Biorad, Hercules, CA) for GCLC and 18s ribosomal RNA. Level of GCLC expression was normalized to the 18s rRNA of the same sample. Fold difference was the ratio of the normalized value of each sample compared to the average 2day results.
Primer Source: Ungvari Z, et al. Adaptive induction of NF-E2-related factor-2-driven antioxidant genes in endothelial cells in response to hyperglycemia. Am J Physiol Heart Circ Physiol April 2011 300:H1133–H1140
Reid G.K., Berardinelli A.J., Ray L., Jackson A.R., Neish A.S., Hansen J.M, & Denning P.W. (2017). Timing of Developmental Reduction in Epithelial Glutathione Redox Potential is Associated with Increased Epithelial Proliferation in the Immature Murine Intestine. Pediatric research, 82(2), 362-369.
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2

Quantitative RT-PCR of GCLC Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols
Proximal small intestine, distal small intestine and colon were isolated from mice of the desired ages into Trizol (Invitrogen, Grand Island, NY). Samples were briefly sonicated and total RNA isolated and reverse transcribed from random hexamer primers using the QuantiTect Reverse Transcription Kit (Qiagen, Carol Stream, IL). The resulting cDNA products were analyzed by real-time quantitative RT-PCR (iQ SYBR Green Supermix on MyiQ real time PCR detection system, Biorad, Hercules, CA) for GCLC and 18s ribosomal RNA. Level of GCLC expression was normalized to the 18s rRNA of the same sample. Fold difference was the ratio of the normalized value of each sample compared to the average 2day results.
Primer Source: Ungvari Z, et al. Adaptive induction of NF-E2-related factor-2-driven antioxidant genes in endothelial cells in response to hyperglycemia. Am J Physiol Heart Circ Physiol April 2011 300:H1133–H1140
Reid G.K., Berardinelli A.J., Ray L., Jackson A.R., Neish A.S., Hansen J.M, & Denning P.W. (2017). Timing of Developmental Reduction in Epithelial Glutathione Redox Potential is Associated with Increased Epithelial Proliferation in the Immature Murine Intestine. Pediatric research, 82(2), 362-369.
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3

Quantifying Intestinal Inflammatory Markers

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Distal small intestinal samples were homogenized and total RNA isolated and reverse transcribed from random hexamer primers using the QuantiTect Reverse Transcription Kit (Qiagen, Carol Stream, IL). The resulting cDNA products were analyzed by real-time quantitative RT-PCR (iQ SYBR Green Supermix on MyiQ real time PCR detection system, Biorad, Hercules, CA) for IL17A, TNFα and GAPDH mRNA). The relative level of mRNA expression for each gene in each sample was normalized to the expression level of reference gene GAPDH and the data were analyzed using the ΔΔCt method [22] (link).
Primer information:
GAPDH-forward: TGG CAA AGT GGA GAT TGT TGC CGAPDH-reverse: AAG ATG GTG ATG GGC TTC CCGIL17A-forward: CAG CAG CGA TCA TCC CTC AAA GIL17A-reverse: CAG GAC CAG GAT CTC TTG CTGTNFα-forward: CTA CTC CCA GGT TCT CTT CAATNFα-reverse: GCA GAG AGG AGG TTG ACT TTC
Weitkamp J.H., Rosen M.J., Zhao Z., Koyama T., Geem D., Denning T.L., Rock M.T., Moore D.J., Halpern M.D., Matta P, & Denning P.W. (2014). Small Intestinal Intraepithelial TCRγδ+ T Lymphocytes Are Present in the Premature Intestine but Selectively Reduced in Surgical Necrotizing Enterocolitis. PLoS ONE, 9(6), e99042.
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