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2 protocols using trisodium citrate

1

Fluorescent Labeling Reagents for Nucleic Acid Visualization

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Sodium dodecylsulfate (SDS, ≥99.0%, Sigma-Aldrich, Germany), n-butanol (>99.5%, Riedel-de-Haën, Germany), α-tocopherol (97%, ABCR, Germany), trisodium citrate (99%, Carl Roth, Germany), gadolinium chloride hexahydrate (>99%, Sigma-Aldrich), irinotecan (98%, ABCR, Germany), sodium adenosine monophosphate (>99%, Sigma-Aldrich, Germany), DUT549P1-aadUTP (Dyomics, Germany), ATTO647N-aadUTP (≥95%, Jena Bioscience, Germany) and fludarabine phosphate (>99%, Sigma-Aldrich, Germany) were used as purchased.
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2

SERS Substrate Fabrication and Analyte Detection

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All chemical compounds employed in this paper were of analytical grade. Silver nitrate ( AgNO3 ) and trisodium citrate were purchased from Roth (Karlsruhe, Germany) and Merck (Darmstadt, Germany), respectively. The aqueous solutions were prepared in Milli-Q water (Milli-Q® Direct Water Purification System, Darmstadt, Germany). MgF2  polished glasses (Crystran Ltd., Poole, UK), having a diameter of 20 mm, were used as port probes for the creation of the solid SERS substrates. The analytes and the pharmaceutical compounds used for SERS measurements (methylene blue MB, doxorubicin DOX, atenolol ATE, metoprolol MET, cysteine Cys and methionine Met) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Cell culture products: Roswell Park Memorial Institute (RPMI 1640) cell culture medium, Fetal Bovine Serum (FBS), Glutamine, Penicillin/Streptomycin and Phosphate Buffered Saline solution (PBS 1X) were purchased from Gibco (Grand Island, NY, USA).
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