Diamond prolong antifade mounting buffer

Manufactured by Thermo Fisher Scientific

Diamond Prolong Antifade mounting buffer is a specialized solution designed to preserve the fluorescence of labeled samples during microscopy. It is formulated to retard the fading of fluorescent dyes, helping to maintain the clarity and integrity of fluorescent images.

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Lab products found in correlation

Bx53 light microscope, by Olympus (2 mentions) Dapi dye, by Thermo Fisher Scientific (2 mentions)

Spelling variants of this product

Prolong Diamond (287 citations) ProLong Diamond Antifade (164 citations) Prolong Antifade (144 citations) Mounting buffer (5 citations) ProLong Diamond Antifade Mounting (4 citations)

2 protocols using diamond prolong antifade mounting buffer

SARS-CoV-2 Nucleocapsid Protein Detection

Cited 2 times

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After fixing for 24 hours in 10% neutral-buffered formalin, mouse tissues were processed, paraffinized, and sectioned to prepare 4 μm tissue sections on glass slides. After dewaxing, antigen retrieval was performed. SARS-CoV-2 nucleocapsid (N) protein was detected by an in-house rabbit polyclonal anti-SARS-CoV-2 N Ab followed by detection with the Alexa488 goat anti-rabbit Ab (Thermo Fisher Scientific). Nuclei were stained with the DAPI dye (Thermo Fisher Scientific) before the sections were mounted with the Diamond Prolong Antifade mounting buffer (Thermo Fisher Scientific) as we previously described (57 (link)). For H&E staining, tissue sections were stained with Gill’s H&E Y (Thermo Fisher Scientific) as we previously described (58 (link)). Images were acquired with the Olympus BX53 light microscope.

Zhang B.Z., Shuai H., Gong H.R., Hu J.C., Yan B., Yuen T.T., Hu Y.F., Yoon C., Wang X.L., Hou Y., Lin X., Huang X., Li R., Au-Yeung Y.M., Li W., Hu B., Chai Y., Yue M., Cai J.P., Ling G.S., Hung I.F., Yuen K.Y., Chan J.F., Huang J.D, & Chu H. (2022). Bacillus Calmette-Guérin–induced trained immunity protects against SARS-CoV-2 challenge in K18-hACE2 mice. JCI Insight, 7(11), e157393.

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SARS-CoV-2 Nucleocapsid Immunofluorescence

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Nucleocapsid of SARS-CoV-2 was detected by the in-house rabbit polyclonal anti-SARS-CoV-2 nucleocapsid antibody as we previously reported [26 ]. Nuclei were stained with the DAPI dye (Thermo Fisher Scientific) before the tissue sections being mounted with the Diamond Prolong Antifade mounting buffer (Thermo Fisher Scientific). Images were acquired with the Olympus BX53 light microscope.

Shuai H., Chan J.F., Yuen T.T., Yoon C., Hu J.C., Wen L., Hu B., Yang D., Wang Y., Hou Y., Huang X., Chai Y., Chan C.C., Poon V.K., Lu L., Zhang R.Q., Chan W.M., Ip J.D., Chu A.W., Hu Y.F., Cai J.P., Chan K.H., Zhou J., Sridhar S., Zhang B.Z., Yuan S., Zhang A.J., Huang J.D., To K.K., Yuen K.Y, & Chu H. (2021). Emerging SARS-CoV-2 variants expand species tropism to murines. EBioMedicine, 73, 103643.

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