600 or 500 mhz spectrometer

Manufactured by Bruker

Sourced in Switzerland

The 600 or 500 MHz spectrometer is a laboratory instrument used for nuclear magnetic resonance (NMR) spectroscopy. It generates a strong magnetic field and radio frequency (RF) pulses to excite and detect the nuclear spins of atoms in a sample, providing information about the molecular structure and dynamics of the sample.

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Lab products found in correlation

Agilent 1100 lc ms, by Agilent Technologies (1 mentions) 6120 quadrupole mass spectrometer, by Agilent Technologies (1 mentions) Zorbax eclipse plus c18 reverse phase column, by Agilent Technologies (1 mentions) 5 mm triple resonance cryoprobe, by Bruker (1 mentions) 3 4 dihydroxybenzaldehyde, by J&K Scientific (1 mentions) Ultraflex maldi tof ms spectrometer, by Bruker (1 mentions) Sodium hypochlorite naclo, by Merck Group (1 mentions) Triethylamine, by Sinopharm (1 mentions) Ferrous sulfate heptahydrate, by Sinopharm (1 mentions) 1200 series, by Agilent Technologies (1 mentions)

4 protocols using 600 or 500 mhz spectrometer

Synthesis and Characterization of Novel Organic Compounds

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All reagents and solvents for the synthesis were purchased from Sigma-Aldrich (USA) and Aladdin (China). The reaction was monitored by column chromatography (200–300 mesh) on silica gel GF254. Melting points were measured and were uncorrected on a Fisher-Johns melting apparatus. Mass spectrometry (MS) was performed by an Agilent 1100 LC-MS (Agilent, Palo Alto, CA, USA).
The ¹H-NMR and ¹³C-NMR spectra data were recorded on a 600 or 500 MHz spectrometer from Bruker Corporation (Switzerland) with TMS as an internal standard.

Chen Q., Zhu M., Xie J., Dong Z., Khushafah F., Yun D., Fu W., Wang L., Wei T., Liu Z., Qiu P., Wu J, & Li W. (2020). Design and Synthesis of Novel Nordihydroguaiaretic Acid (NDGA) Analogues as Potential FGFR1 Kinase Inhibitors With Anti-Gastric Activity and Chemosensitizing Effect. Frontiers in Pharmacology, 11, 518068.

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NMR and HPLC-MS Analysis of Organic Compounds

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All ¹H, ¹³C, and two-dimensional (¹H–¹H correlation spectroscopy [COSY], heteronuclear single quantum coherence [HSQC] spectroscopy, and heteronuclear multiple-bond correlation [HMBC] spectroscopy) NMR spectra were acquired on a Bruker 600- or 500-MHz spectrometer equipped with a 5-mm triple-resonance cryoprobe at 298 K. Residual solvent signals were used as references (acetone-d₆, δ_H 2.05/δ_C 29.8, 206.0, and CDCl₃, δ_H 7.26/δ_C 77.0). HPLC-MS spectra were obtained on an Agilent 6120 quadrupole mass spectrometer using a positive ESI source. Amounts of 10 µl of the test samples were injected for HPLC-MS analysis (Agilent Zorbax Eclipse plus C₁₈ reverse-phase column, 5 µm, 4.6 by 150 mm, 10% to 90% CH₃CN in H₂O with 01% formic acid for 30 min, 1 ml·min⁻¹). HRESIMS data were obtained using the Agilent 6520 quadrupole time of flight (Q-TOF) LC-MS instrument equipped with an electrospray ionization (ESI) source.

Yue Q., Chen L., Li Y., Bills G.F., Zhang X., Xiang M., Li S., Che Y., Wang C., Niu X., An Z, & Liu X. (2015). Functional Operons in Secondary Metabolic Gene Clusters in Glarea lozoyensis (Fungi, Ascomycota, Leotiomycetes). mBio, 6(3), e00703-15.

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Synthesis of Fluorescent Benzaldehyde Derivatives

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3,4-Dihydroxybenzaldehyde, 1-bromohexadecane, boron trifluoride diethyletherate (BF₃·Et₂O), trifluoroacetic acid and 2-(diphenylphosphino)benzaldehyde were purchased from J&K Scientific Ltd. 2,4-Dimethyl-pyrrole was obtained from Nanjing Chemlin Chemical Industry Co. Ltd. Sodium hypochlorite (NaClO) and potassium dioxide (KO₂), tert-butyl hydroperoxide (TBHP) were supplied by Sigma Aldrich. Other chemicals or solvents were purchased from Sinopharm Chemical Reagent Co. Ltd, such as hydrogen peroxide (H₂O₂), ferric chloride hexahydrate (FeCl₃·6H₂O), ferrous sulfateheptahydrate (FeSO₄·7H₂O), N,N-dimethylformamide (DMF), dimethyl sulfoxide (DMSO), triethylamine (Et₃N), dichloromethane (CH₂Cl₂), et al. All chemicals and solvents were used directly without further purification unless otherwise noted. All air and moisture sensitive reactions were carried out under an argon atmosphere. Dry CH₂Cl₂ was obtained by refluxing and distilling over CaH₂ under nitrogen. triethylamine was obtained by simple distillation.
Nuclear magnetic resonance (NMR) spectra were obtained from a Bruker 500 or 600 MHz spectrometer with TMS as an internal standard operating for ¹H, ¹³C NMR and ³¹P NMR. Mass spectra were measured with a Bruker ultraflex MALDI TOF MS spectrometer. HRMS data were obtained on an Agilent 6224.

Qu X., Bian Y., Chen Y, & Wei X. (2020). A sensitive BODIPY-based fluorescent probe for detecting endogenous hydroxyl radicals in living cells. RSC Advances, 10(48), 28705-28710.

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NMR Spectroscopy Characterization Protocol

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NMR spectral data were recorded at room temperature using a Bruker 500 or 600 MHz spectrometer unless stated otherwise. The NMR data are reported as follows: chemical shifts in ppm from an internal tetramethylsilane standard on the δ scale, multiplicity (br=broad, app=apparent, s=singlet, d=doublet, t=triplet, q=quartet, and m=multiplet), coupling constants (Hz), and integration. Analytical thin layer chromatography (TLC) was performed using EMD Reagents 0.25 mm silica gel 60-F plates. “Flash” chromatography on silica gel was performed using Silicycle silica gel (40–63 μm). All reactions were carried out under an atmosphere of nitrogen in glassware that was evacuated and back-filled with nitrogen three times. Reactions were carried out at room temperature unless otherwise indicated. Unless otherwise noted, all reagents were commercially obtained and, where appropriate, purified prior to use. THF, Et₂O, DMF and CH₂Cl₂ were dried by filtration through alumina according to the procedure of Grubbs and coworkers.^{[78 ]} For final compounds the purity was determined by HPLC (Agilent Technologies series 1200).

Premachandra I.D., Scott K.A., Shen C., Wang F., Lane S., Liu H, & Van Vranken D.L. (2015). Potent Synergy between Spirocyclic Pyrrolidinoindolinones and Fluconazole against Candida albicans. ChemMedChem, 10(10), 1672-1686.

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