C4-2 and CWR22Rν1 cells were seeded on a two-chamber tissue culture glass slides and treated with 40μg/ml of MEM at 70% confluence for 24h. After washing with PBS cells were fixed with 2% paraformaldehyde followed by permeabilization with methanol and blocking with 2% serum. Incubation with primary antibodies overnight was followed by incubation with appropriate fluorophore tagged secondary antibodies. Antifade DAPI (Invitrogen, NY) was used as mounting and counterstaining medium. For analysis, Bio-Rad Radiance 2100 MP Rainbow system for biological imaging was used. To detect apoptotic and necrotic cells the Annexin-V-Fluos Staining Kit (Roche, Switzerland) was used according to the manufacturer’s protocol. Zeiss LSM 410 confocal microscopy was used to measured fluorescence. The cells stained with Annexin-V and unstained cells in a selected field were counted to ascertain the extent of apoptosis and necrosis.
Radiance 2100 mp rainbow
Manufactured by Bio-Rad
Sourced in Germany
The Radiance 2100 MP Rainbow is a multi-parameter flow cytometer. It is designed to analyze and sort cells based on their physical and fluorescent characteristics.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using radiance 2100 mp rainbow
1
Apoptosis and Necrosis Assay
2
Confocal Imaging of Protein Localization
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Immunofluorescent labelling of all proteins was visualised using laser scanning confocal microscopes (TCS SP [Leica Microsystems, Heidelberg, Germany], LSM 5 [Carl Zeiss, Oberkochen, Germany], or Radiance 2100 MP Rainbow [Bio-Rad, Philadelphia, PA]: made available by the Division of Research Instrument and Equipment, Life Science Research Centre, Kagawa University) to detect FITC signals. Images were collected from a single optical slice through the centre of each myocyte. More than 10 cells were observed from 3 hearts for each protein investigated and at least 3 typical images (i.e., from 3 cells for each protein investigated) were saved for labelling analysis. No labelling was detectable without either the primary or secondary IgGs.
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