Ms73 probe

Manufactured by Bandelin

Sourced in Germany

The MS73 probe is a laboratory equipment designed for underwater ultrasonic cleaning. It is a component used in ultrasonic cleaning systems, providing the necessary transducer technology to generate the ultrasonic waves required for the cleaning process.

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Lab products found in correlation

Sonoplus hd 2070, by Bandelin (4 mentions) Cell strainer, by Avantor (1 mentions) Sonopuls hd 2200, by Bandelin (1 mentions) Illustra nap 25 columns, by GE Healthcare (1 mentions) His spintrap column, by GE Healthcare (1 mentions) Infinite f200, by Tecan (1 mentions)

6 protocols using ms73 probe

Tissue Homogenization and Antioxidant Analysis

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Portions of liver and kidney tissue, 75–100 mg were homogenized in 1 mL of 50 mM phosphate buffer (pH 7.0) by ultrasonic homogenizer SONOPLUS Bandelin HD2070 (Bandelin, Germany) using the MS73 probe (Bandelin, Germany) with 10% power. Homogenates were centrifuged by Micro 200R centrifuge (Hettich, Germany) for 15 minutes at a speed of 10 000 × g at +4°C. The supernatant was used for the measurements of glutathione and lipid peroxidation level, as well as catalase (CAT) activity. All methods are described in previously published work by Brzovic Saric et al. [29 (link)].
All parameters normalized in relation to exact protein content.

Ilić I., Oršolić N., Rođak E., Odeh D., Lovrić M., Mujkić R., Delaš Aždajić M., Grgić A., Tolušić Levak M., Vargek M., Dmitrović B, & Belovari T. (2020). The effect of high-fat diet and 13-cis retinoic acid application on lipid profile, glycemic response and oxidative stress in female Lewis rats. PLoS ONE, 15(9), e0238600.

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Analyzing Liver Lipid Parameters

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Blood was collected by cardiac puncture and centrifuged to isolate serum for analysis of lipid parameters. The serum was immediately frozen at −80 °C until analysis. Livers were isolated and parts of tissue samples were placed in 50 mmol/L phosphate-buffered saline (PBS, pH = 7.4) and homogenized (10% of homogenate by tissue mass per volume of PBS) with an ultrasonic homogenizer (SONOPLUS HD2070, Bandelin Electronic GmbH & Co KG, Hagen, Germany) with an MS73 probe (Bandelin, Electronic, Hagen, Germany). Sonication of liver homogenates was performed on ice for 30 s in three 10 s intervals. Homogenates were centrifuged at 4 °C and 20,000× g for 15 min, and supernatants were separated and immediately frozen at −80 °C until the analysis of PPARs, ACOX1, and oxidative stress parameters.

Domjanić Drozdek S., Odeh D., Đikić D., Gračan R., Oršolić N., Dragović-Uzelac V., Feher-Turković L., Dragičević P, & Landeka Jurčević I. (2022). The Effects of Nettle Extract Consumption on Liver PPARs, SIRT1, ACOX1 and Blood Lipid Levels in Male and Female C57Bl6 Mice. Nutrients, 14(21), 4469.

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Liver Tissue Extraction and Analysis

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Blood was collected by cardiac puncture, and animals sacrificed by cervical dislocation. Collected blood was centrifuged to isolate serum for biochemical parameters. Serum was immediately frozen at -80 °C until analysis. Livers were isolated and parts of tissue samples placed in 50 mmol/L phosphate-buffered saline (PBS, pH=7.4) and homogenised (10 % of homogenate by tissue mass per volume of PBS) with an ultrasonic homogeniser (SONOPLUS HD2070, BANDELIN electronic, Berlin Germany) using an MS73 probe (BANDELIN). The homogenates were then sonicated on ice for 30 s in three 10-second intervals and centrifuged at 4 °C and 20000×g for 15 min and immediately frozen at -80 °C until the analysis of oxidative stress parameters and transcription factors. Small liver samples for histological examination were frozen without any chemical treatment immediately after dissection and used for cryostat histology.

Jutrić D., Đikić D., Boroš A., Odeh D., Drozdek S.D., Gračan R., Dragičević P., Crnić I, & Jurčević I.L. (2022). Effects of Naringin and Valproate Interaction on Liver Steatosis and Dyslipidaemia Parameters in Male C57BL6 Mice. Archives of Industrial Hygiene and Toxicology, 73(1), 71-82.

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Activated Sludge Cell Isolation Protocol

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Activated sludge (30 mL) from aeration tanks was obtained at the AAW WWTP on 16 December 2020 and 03 March 2021 and immediately homogenized in the lab using the RZR 2020 Benchtop Stirrer (Heidolph, Schwabach, Germany) with a glass/Teflon tissue grinder attached (1 min, 2nd gear). Five milliliters was subsequently sonicated using a Bandelin Sonopuls HD2200 with an MS73 probe (Berlin, Germany) set at 60% amplitude with 6 × 10 s pulses with a 10-s interval. Single cells were separated by centrifugation through a cell strainer with pore sizes of 40 µm (VWR) for 5 min at 3,000 × g, followed by centrifugation through a cell strainer with pore sizes of 5 µm (pluriSelect Life Science, Leipzig, Germany) for 2 min at 8,600 × g. Cells present in the permeate were counted in a Bürker-Türk counting chamber and subsequently diluted to approx. 10,000 cells/mL.

Verhoeven M.D., Nielsen P.H, & Dueholm M.K. (2023). Amplicon-guided isolation and cultivation of previously uncultured microbial species from activated sludge. Applied and Environmental Microbiology, 89(12), e01151-23.

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Bacterial Protein Expression and Purification

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For protein expression, bacteria were grown in the dark on plates with LB-agar supplemented with ampicillin for 3 days at room temperature. To measure crude extracts, we resuspended the bacteria in 20 mM MOPS pH 7.2, sonicated the suspension with an MS 73 probe (Bandelin) and centrifuged the sample with a tabletop centrifuge (Eppendorf). Protein extraction was performed with His Spin trap columns according to the manufacturer’s instructions (GE Healthcare). We resuspended the bacteria of one Petri dish in 2 ml binding buffer and sonicated with a MS 73 probe. Buffer exchange was performed with illustra NAP-25 columns (GE Healthcare). All measurements were performed in 20 mM MOPS (Sigma-Aldrich) with an Infinite F200 plate reader (Tecan).

Herud-Sikimić O., Stiel A.C., Kolb M., Shanmugaratnam S., Berendzen K.W., Feldhaus C., Höcker B, & Jürgens G. (2021). A biosensor for the direct visualization of auxin. Nature, 592(7856), 768-772.

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Tissue Harvesting and Preparation for Bioanalysis

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At designated experimental days for organ collection, animals were anesthetized by halothane and perfused through with 10 mL of phosphor buffer saline (PBS) and sacrificed by cervical dislocation, 24 h after the last administered dose on the particular day of experiment. Intestine, liver, and kidneys were extracted. Such tissue samples were used for antioxidative activity assays and for the determination of individually bioaccumulated phenolics by the UPLC MS/MS method. Prior to the measurement of antioxidative parameters and UPLC-MS/MS analysis, the tissue samples were placed in 50 mM phosphate buffer (pH=7.4) and homogenized (10% of homogenate, by tissue mass per volume of PBS) with an ultrasonic homogenizer (SONOPLUS Bandelin HD2070, Bandelin Electronic GmbH & Co KG, Germany) using an MS73 probe (Bandelin, Electronic GmbH & Co KG Germany). Thereafter, homogenates were sonicated on ice for 30 s in three 10-s intervals, centrifuged at 20,000×g for 15 min at 4°C, and immediately frozen at -80°C until analysis. Further details of supernatant treatment for antioxidative activity determination or UPLC-MS/MS analysis are described in each section separately.

Balta V., Đikić D., Crnić I., Odeh D., Oršolić N., Kmetič I., Murati T., Dragović‐Uzelac V., & Jurčević I.L. (2020). Effects of Four-Week Intake of Blackthorn Flower Extract on Mice Tissue Antioxidant Status and Phenolic Content. Polish Journal of Food and Nutrition Sciences.

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