Ap20545pu n

Manufactured by Santa Cruz Biotechnology

AP20545PU-N is a laboratory instrument designed for use in scientific research. It is a high-quality product manufactured by Santa Cruz Biotechnology. The core function of this equipment is to perform various analytical tasks required in a research laboratory setting. However, a more detailed description cannot be provided while maintaining an unbiased and factual approach without extrapolating on the intended use.

Automatically generated - may contain errors

Lab products found in correlation

Okadaic acid, by Merck Group (2 mentions) Pser10 histone h3, by Merck Group (2 mentions) Irdye800 conjugated anti mouse and anti rabbit, by Rockland Immunochemicals (2 mentions) Anti mouse cy3 conjugated antibody, by Thermo Fisher Scientific (2 mentions) Forskolin, by Merck Group (2 mentions) Myc tag, by Merck Group (2 mentions) Tautomycetin, by Bio-Techne (2 mentions) β actin, by Merck Group (2 mentions) Caffeine, by Merck Group (2 mentions) Tetradecanoyl phorbol acetate, by Merck Group (1 mentions) Haloperidol, by Merck Group (1 mentions) A5316, by Merck Group (1 mentions) Quinpirole hydrochloride, by Merck Group (1 mentions)

3 protocols using ap20545pu n

Phosphorylation and Regulation of Neuronal Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols

Primary antibodies were: mouse monoclonal for DARPP-32 5a and 6 (ref. 58 (link); 1/5,000), calmodulin (Upstate Cell Signaling # 05–173); rabbit polyclonal for pThr34, pThr75 and pSer97 (#12438, #2301, #3401; 1:2,000; Cell Signaling); total β-adducin (#AP20545PU-N, 1:500; Santa Cruz, Acris); pSer713 of β-adducin (also reacts with pSer662 of γ-adducin and pSer724 of α-adducin; #05-587; 1:1,000; Chemicon/Millipore); β-actin (#A5316; 1:1,000; Sigma); myc-tag (#05-724; 1:500; Millipore); GFP (Abcam #ab6556, 1/1,000); pThr79-pSer81-calmodulin (Abcam, #ab194526); and pSer10 histone H3 (#06-570; rabbit; Millipore). Secondary antibodies comprised of IRDye800-conjugated anti-mouse and anti-rabbit (#610-132-121, #611-132-122; both 1:4,000; Rockland) antibodies for immunoblotting and anti-mouse CY3-conjugated antibody (#A10521; 1:400; Molecular Probes) for immunochemistry. COS-7 cells were treated with one or the combination of the following compounds, as indicated, or with dimethyl sulfoxide vehicle: Sp 5,6-DCl-cBIMPS (10 μM; BIOLOG Life Science); forskolin (100 μM; Sigma); tetradecanoylphorbol-acetate (100 nM; Sigma); okadaic acid (200 nM; Sigma); and tautomycetin (10 nM; Tocris). Mice were injected intraperitoneally with the following drugs or with their vehicle (9 g l⁻¹ NaCl) caffeine (7.5 mg kg⁻¹, Sigma) and cocaine (10 or 20 mg kg⁻¹, Coper).

Engmann O., Giralt A., Gervasi N., Marion-Poll L., Gasmi L., Filhol O., Picciotto M.R., Gilligan D., Greengard P., Nairn A.C., Hervé D, & Girault J.A. (2015). DARPP-32 interaction with adducin may mediate rapid environmental effects on striatal neurons. Nature Communications, 6, 10099.

+ Open protocol

+ Expand

Protein Phosphorylation Quantification

Check if the same lab product or an alternative is used in the 5 most similar protocols

Primary antibodies were: Mouse monoclonal for DARPP-32 5a and 6^{58 (link)} (1/5,000), calmodulin (Upstate Cell Signaling # 05-173); rabbit polyclonal for pThr34, pThr75, pSer97 (#12438, #2301, #3401; 1:2,000; Cell Signaling); total β-adducin (#AP20545PU-N, 1:500; Santa Cruz, Acris), pSer713 of β-adducin (also reacts with pSer662 of γ-adducin and pSer724 of α-adducin; #05-587; 1:1,000; Chemicon/Millipore); β-actin (#A5316; 1:1,000; Sigma); myc-tag (#05-724; 1:500; Millipore), GFP (Abcam #ab6556, 1/1,000), pThr79-pSer81-calmodulin (Abcam, #ab194526), pSer10 histone H3 (#06-570; rabbit; Millipore). Secondary antibodies comprised IRDye800-conjugated anti-mouse and anti-rabbit (#610-132-121, #611-132-122; both 1:4,000; Rockland) antibodies for immunoblotting and anti-mouse CY3-conjugated antibody (#A10521; 1:400; Molecular Probes) for immunochemistry. COS-7-cells were treated with one or the combination of the following compounds, as indicated, or with DMSO-vehicle: Sp 5,6-DCl-cBIMPS (10 μM; BIOLOG life science), forskolin (100 μM; Sigma), tetradecanoylphorbol-acetate (TPA, 100 nM; Sigma), okadaic acid (200 nM; Sigma), tautomycetin (10 nM; Tocris). Mice were injected intraperitoneally with the following drugs or with their vehicle (9 g l⁻¹ NaCl) caffeine (7.5 mg kg⁻¹, Sigma) and cocaine (10 or 20 mg kg⁻¹, Coper).

+ Open protocol

+ Expand

Spine Morphology and Signaling Pathways

Check if the same lab product or an alternative is used in the 5 most similar protocols

Mice were injected intraperitoneally (IP) with the following drugs or with their vehicle (9 g/l NaCl): quinpirole hydrochloride (0.2 mg/kg, Sigma, 15 min for biochemistry, 24 h for Golgi-Cox), haloperidol (0.5 mg/kg, Sigma, 1 h for biochemistry, 24 h for Golgi-Cox) and caffeine (7.5 mg/kg, Sigma, 40 min for biochemistry, 24 h for Golgi-Cox). Spine morphology was studied at 24 h because we have previously shown spine changes after 24h NEE. Primary antibodies were for total β-adducin (#AP20545PU-N, 1:500; Santa Cruz, Acris), PKC-phosphorylated adducins (corresponding to pSer713 in β-adducin, #05-587; 1:1,000; Chemicon/Millipore), mouse monoclonal for DARPP-32 (1:5,000, Cell Signaling Technology), rabbit polyclonal for pThr34, pThr75, pSer97 (#12438, #2301, #3401; 1:2,000; Cell Signaling), and β-actin (#A5316; 1:1,000; Sigma). Secondary antibodies were anti-mouse CY3-conjugated antibody (#A10521; 1:400; Molecular Probes) for immunochemistry and IRDye800-conjugated anti-mouse and anti-rabbit (#610-132-121, #611-132-122 ; both 1:4,000; Rockland) antibodies for immunoblotting.

Engmann O., Giralt A, & Girault J.A. (2016). Acute drug-induced spine changes in the nucleus accumbens are dependent on β-adducin. Neuropharmacology, 110(Pt A).

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!