Recombinant pifn γ

Manufactured by R&D Systems

Recombinant pIFN-γ is a laboratory product produced by R&D Systems. It is a recombinant form of the human interferon gamma protein.

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Lab products found in correlation

Jm1e3, by Bio-Rad (1 mentions) Htnf α, by Merck Group (1 mentions) Y 27632, by Merck Group (1 mentions)

2 protocols using recombinant pifn γ

Activation of Porcine Aortic Endothelial Cells

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pAECs were collected from wild-type pig aortas, and cultured as previously described [36 (link)]. pAECs of passages 3 to 5 were used as stimulators for the mixed lymphocyte reaction (MLR). The sub-confluent pAECs were activated for 72h by co-culture with recombinant pIFN-γ (50ng/mL, R&D Systems, Minneapolis, MN). Activation of the cells was evaluated by staining with swine leukocyte antigen (SLA) class I (mouse anti-pig SLA class I, clone JM1E3, Serotec, Raleigh, NC) and SLA class II (DR) (mouse anti-pig SLA class II, clone 2E9/13, BD Biosciences, San Jose, CA) using flow cytometry [38 (link)].

Lee W., Satyananda V., Iwase H., Tanaka T., Miyagawa Y., Long C., Ayares D., Cooper D.K, & Hara H. (2015). IN VITRO TESTING OF AN ANTI-CD40 MONOCLONAL ANTIBODY, CLONE 2C10, IN PRIMATES AND PIGS. Transplant immunology, 33(3), 185-191.

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Activation of pCECs and Monocyte Assay

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pCECs were cultured, as previously described ^{11 (link)}. pCECs between passage 2 to 4 were used in all experiments. Subconfluent pCECs were activated by recombinant pIFN-γ (50ng/mL, R&D system, Minneapolis, MN) or hTNF-α (50ng/mL, Serotec, Raleigh, NC) for adequate periods depending on the objectives of the study. Supernatants obtained from pCECs culture with/without Y27632 (Sigma-Aldrich, St. Louis, MO) 6 hours after hTNF-α stimulation were collected for quantification of monocyte chemotactic protein-1 (MCP-1), and monocyte migration assay.

Lee W., Miyagawa Y., Long C., Zhang M., Cooper D.K, & Hara H. (2015). EFFECT OF RHO-KINASE INHIBITOR, Y27632, ON PORCINE CORNEAL ENDOTHELIAL CELL CULTURE, INFLAMMATION AND IMMUNE REGULATION. Ocular immunology and inflammation, 24(5), 579-593.

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