The largest database of trusted experimental protocols

Recombinant pifn γ

Manufactured by R&D Systems

Recombinant pIFN-γ is a laboratory product produced by R&D Systems. It is a recombinant form of the human interferon gamma protein.

Automatically generated - may contain errors

2 protocols using recombinant pifn γ

1

Activation of Porcine Aortic Endothelial Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
pAECs were collected from wild-type pig aortas, and cultured as previously described [36 (link)]. pAECs of passages 3 to 5 were used as stimulators for the mixed lymphocyte reaction (MLR). The sub-confluent pAECs were activated for 72h by co-culture with recombinant pIFN-γ (50ng/mL, R&D Systems, Minneapolis, MN). Activation of the cells was evaluated by staining with swine leukocyte antigen (SLA) class I (mouse anti-pig SLA class I, clone JM1E3, Serotec, Raleigh, NC) and SLA class II (DR) (mouse anti-pig SLA class II, clone 2E9/13, BD Biosciences, San Jose, CA) using flow cytometry [38 (link)].
+ Open protocol
+ Expand
2

Activation of pCECs and Monocyte Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
pCECs were cultured, as previously described 11 (link). pCECs between passage 2 to 4 were used in all experiments. Subconfluent pCECs were activated by recombinant pIFN-γ (50ng/mL, R&D system, Minneapolis, MN) or hTNF-α (50ng/mL, Serotec, Raleigh, NC) for adequate periods depending on the objectives of the study. Supernatants obtained from pCECs culture with/without Y27632 (Sigma-Aldrich, St. Louis, MO) 6 hours after hTNF-α stimulation were collected for quantification of monocyte chemotactic protein-1 (MCP-1), and monocyte migration assay.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!