For immunofluorescence, cells and heart sections were blocked with normal goat serum for 40 min, and then incubated with primary antibodies overnight at 4 °C, followed by incubation with secondary antibodies (1:500 dilution) to protect from the light at room temperature for 1 h. The following primary antibodies were used: anti-Sca-1 (1:100, Santa Cruz), anti-Islet-1 (1:300, Abcam), Nkx2.5 (1:100, Santa Cruz), and GATA4 (1:100, Santa Cruz). The following secondary antibodies were used: Alexa Fluor Chicken-anti-Rat 647, Alexa Fluor Goat-anti-Mouse 488, Alexa Fluor Chicken-anti-Rat 594, and Alexa Fluor Goat-anti-Rabbit 594 (Invitrogen). Microscopic examination was performed and was randomly selected from each chamber. All images were obtained using a LSM-780 confocal microscope and were analyzed using ImageJ software.
Anti sca 1
Manufactured by Santa Cruz Biotechnology
Anti-Sca-1 is a laboratory reagent used for the detection and identification of Sca-1 (stem cell antigen-1) in biological samples. Sca-1 is a cell surface marker expressed on various stem and progenitor cells. The Anti-Sca-1 product can be used in flow cytometry, immunohistochemistry, and other applications to analyze the presence and distribution of Sca-1-positive cells.
Automatically generated - may contain errors
Lab products found in correlation
Gata4, by Santa Cruz Biotechnology (1 mentions)
Alexa fluor 488 goat anti mouse, by Thermo Fisher Scientific (1 mentions)
Anti islet 1, by Abcam (1 mentions)
Alexa fluor 594 goat anti rabbit, by Thermo Fisher Scientific (1 mentions)
Aspirin, by Merck Group (1 mentions)
Wild type sprague dawley rats, by Charles River Laboratories (1 mentions)
Prasugrel, by Merck Group (1 mentions)
Anti ki67, by Santa Cruz Biotechnology (1 mentions)
Mouse c reactive protein crp elisa kit, by R&D Systems (1 mentions)
Anti sca 1, by Abcam (1 mentions)
Anti nkx2, by Abcam (1 mentions)
Anti ki67, by Abcam (1 mentions)
Anti cd105, by Abcam (1 mentions)
Anti oct4, by Santa Cruz Biotechnology (1 mentions)
Trizol reagent, by Thermo Fisher Scientific (1 mentions)
Anti β actin, by Merck Group (1 mentions)
Anti cd31, by Abcam (1 mentions)
Ticagrelor, by AstraZeneca (1 mentions)
2 protocols using anti sca 1
1
Immunofluorescence Staining of Cardiac Markers
2
Rat Cardiac Regeneration Evaluation
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Wild-type Sprague-Dawley rats were purchased from Charles River Laboratories (Wilmington, MA). Ticagrelor was provided by AstraZeneca. Aspirin and prasugrel were purchased from Sigma.
Mouse C-Reactive Protein/CRP ELISA Kit was purchased from R&D systems. Myocardial PGE 2, 6-keto-PGF 1α and 15-epi-lipoxin A 4 ELISA kits were purchased from Cayman Chemical. TRIzol reagent was purchased from Invitrogen, Carlsbad, CA. Anti-Ki-67, c-Kit, anti-Oct4 and anti-Sca-1 antibodies for the immunoblotting were purchased from Santa Cruz Biotechnology and anti-β-actin from Sigma-Aldrich. Anti-CD31, Anti-Ki-67, anti-CD105, anti-Sca-1 and anti-Nkx2.5 antibodies for the immunohistochemistry were purchased from Abcam. Anti-CD 31 http://www.abcam.com/cd31-antibody-tld-3a12-ab64543. htmland anti-c-Kit antibodies for the immunofluorescence staining were purchased from Abcam and the fluorescence-labeled secondary antibodies (AlexaFluor 488 for CD31 and AlexaFluor 546 for c-Kit) were purchased from Invitrogen.
Mouse C-Reactive Protein/CRP ELISA Kit was purchased from R&D systems. Myocardial PGE 2, 6-keto-PGF 1α and 15-epi-lipoxin A 4 ELISA kits were purchased from Cayman Chemical. TRIzol reagent was purchased from Invitrogen, Carlsbad, CA. Anti-Ki-67, c-Kit, anti-Oct4 and anti-Sca-1 antibodies for the immunoblotting were purchased from Santa Cruz Biotechnology and anti-β-actin from Sigma-Aldrich. Anti-CD31, Anti-Ki-67, anti-CD105, anti-Sca-1 and anti-Nkx2.5 antibodies for the immunohistochemistry were purchased from Abcam. Anti-CD 31 http://www.abcam.com/cd31-antibody-tld-3a12-ab64543. htmland anti-c-Kit antibodies for the immunofluorescence staining were purchased from Abcam and the fluorescence-labeled secondary antibodies (AlexaFluor 488 for CD31 and AlexaFluor 546 for c-Kit) were purchased from Invitrogen.
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